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| THE MOLECULAR-WEIGHT CUTOFF OF MICROCAPSULES IS DETERMINED BY THE REACTION BETWEEN ALGINATE AND POLYLYSINE | |
| Vandenbossche, G. M. R. | |
| 1993 | |
| Source Publication | Biotechnology and Bioengineering
 |
| ISSN | 0006-3592 |
| Volume | 42Issue:3Pages:381-386 |
| Abstract | Mammalian cells encapsulated in alginate-polylysine microcapsules are used as artificial organs in cancer research and in biotechnology. These applications require microcapsules with a reproducible mol. wt. cut-off. The high cost of the polycation, polylysine, requires an efficient preparation procedure. This article shows that the overall reported contact time of 5 minutes at ambient conditions should be increased several times in order to reach a maximal binding between the calcium alginate beads and 0.1% (w/v) polylysine solutions. An increase of the polylysine concentration from 0.0125% to 0.8% (w/v) resulted in a faster maximal binding, but the amount of polylysine bound increased also. Immersion of calcium alginate beads with a diameter of 750 mum, prepared from 1 mL alginate, in 30 mL of a 0.8% (w/v) polylysine solution, resulted in a polylysine spill of more than 89%. The time required to reach a maximal binding was related to the reaction temperature. The interaction zone between calcium alginate beads and fluorescein isothiocyanate-labeled polylysine solutions was visualized with a confocal laser scanning microscope as a function of time. Microcapsules, prepared at 40-degrees-C with 0.1% (w/v) polylysine solutions with mol. wts. between 12 and 249.2 kD, were permeable for fluorescein isothiocyanate-labeled dextran, mol. wt. 4.7, but not for 40.5 kD. Higher polylysine concentrations resulted in a membrane with a mol. wt. cut-off lower than 4.7 kD. |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8884 |
| Collection | 公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| Recommended Citation GB/T 7714 | Vandenbossche, G. M. R.. THE MOLECULAR-WEIGHT CUTOFF OF MICROCAPSULES IS DETERMINED BY THE REACTION BETWEEN ALGINATE AND POLYLYSINE[J]. Biotechnology and Bioengineering,1993,42(3):381-386. |
| APA | Vandenbossche, G. M. R..(1993).THE MOLECULAR-WEIGHT CUTOFF OF MICROCAPSULES IS DETERMINED BY THE REACTION BETWEEN ALGINATE AND POLYLYSINE.Biotechnology and Bioengineering,42(3),381-386. |
| MLA | Vandenbossche, G. M. R.."THE MOLECULAR-WEIGHT CUTOFF OF MICROCAPSULES IS DETERMINED BY THE REACTION BETWEEN ALGINATE AND POLYLYSINE".Biotechnology and Bioengineering 42.3(1993):381-386. |
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| THE MOLECULAR-WEIGHT（661KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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