Institutional Repository of Xishuangbanna Tropical Botanical Garden
| Agrobacterium tumefaciens-mediated transformation protocol of Jatropha curcas L. using leaf and hypocotyl segments | |
| Misra, P. | |
| 2012 | |
| Source Publication | Journal of Plant Biochemistry and Biotechnology
 |
| ISSN | 0971-7811 |
| Volume | 21Issue:1Pages:128-133 |
| Abstract | In the present study, Agrobacterium tumefaciens-mediated transformation protocol has been optimized for Jatropha curcas-an important biofuel plant using leaf segments (LS) and hypocotyl segments (HS) from in vitro-seedlings. Among different accessions used, 'GJ-Ran-C2' was selected as the best for transformation. A. tumefaciens strain EHA101, containing the binary vector pIG121Hm was used for transformation, along with the GUS reporter gene containing intron and CaMV 35S as promoter. Various parameters were optimized to get the highest transient transformation expression (TTE). AugmentinA (R) was used in the medium to control A. tumefaciens. Selection of transgenic shoots was done in the presence of kanamycin. Integration of GUS gene was confirmed by PCR analysis, whereas the expression of GUS transgene was confirmed by RT-PCR, using actin gene as internal control. GUS histochemical analysis of explants just after cocultivation showed blue color in approximately all the LS and HS but stable transformation efficiency was 5% in case of LS, whereas 4% in HS. |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8018 |
| Collection | 热带植物资源可持续利用重点实验室特色文献_小桐子相关文献 |
| Recommended Citation GB/T 7714 | Misra, P.. Agrobacterium tumefaciens-mediated transformation protocol of Jatropha curcas L. using leaf and hypocotyl segments[J]. Journal of Plant Biochemistry and Biotechnology,2012,21(1):128-133. |
| APA | Misra, P..(2012).Agrobacterium tumefaciens-mediated transformation protocol of Jatropha curcas L. using leaf and hypocotyl segments.Journal of Plant Biochemistry and Biotechnology,21(1),128-133. |
| MLA | Misra, P.."Agrobacterium tumefaciens-mediated transformation protocol of Jatropha curcas L. using leaf and hypocotyl segments".Journal of Plant Biochemistry and Biotechnology 21.1(2012):128-133. |
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| Agrobacterium tumefa（270KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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