Institutional Repository of Xishuangbanna Tropical Botanical Garden
| Isolation of High Quality RNA from Oilseeds of Jatropha curcas | |
| Singh, R. K. | |
| 2009 | |
| Source Publication | Journal of Plant Biochemistry and Biotechnology
 |
| ISSN | 0971-7811 |
| Volume | 18Issue:1Pages:77-81 |
| Abstract | High quality RNA with good yield is a prerequisite for carrying out several molecular biology studies. Recalcitrant tissues such as oilseeds pose several problems while isolating good quality RNA. We have standardized a fast and simple protocol for RNA isolation from the seeds of Jatropha curcas, which gives good quality RNA without compromising for the yield. by including pre wash of seed powder with acetone and removal of polysaccharides through selective precipitation, we have been regularly isolating good quality total RNA in the range of 300-450 mu g g(-1) depending upon tissue type. The RNA isolated by this procedure is devoid of any contaminating DNA. The RNA preparations have been subjected to cDNA synthesis and PCR, and found suitable for these studies. This method also works satisfactorily with groundnut and mustard seeds. |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/7704 |
| Collection | 热带植物资源可持续利用重点实验室特色文献_小桐子相关文献 |
| Recommended Citation GB/T 7714 | Singh, R. K.. Isolation of High Quality RNA from Oilseeds of Jatropha curcas[J]. Journal of Plant Biochemistry and Biotechnology,2009,18(1):77-81. |
| APA | Singh, R. K..(2009).Isolation of High Quality RNA from Oilseeds of Jatropha curcas.Journal of Plant Biochemistry and Biotechnology,18(1),77-81. |
| MLA | Singh, R. K.."Isolation of High Quality RNA from Oilseeds of Jatropha curcas".Journal of Plant Biochemistry and Biotechnology 18.1(2009):77-81. |
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| Isolation of High Qu（99KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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