| Pretreatment incubation for culture and cryopreservation of Sabal embryos | |
| Wen, B; Wang, RL | |
| 2010 | |
| Source Publication | PLANT CELL TISSUE AND ORGAN CULTURE
 |
| Volume | 102Issue:2Pages:237-243 |
| Abstract | Zygotic embryos from mature seeds of Sabal jamaicensis, S. minor, S. umbraculifera and S. yapa were cultured in vitro and cryopreserved successfully. Seed pretreatment prior to embryo isolation was shown to be crucial. Soaking seeds in water or sowing on 1% agar followed by incubation at 30A degrees C for 1 day (S. jamaicensis, S. minor and S. umbraculifera) or 3 days (S. yapa only) prior to embryo isolation increased the percentage emergence of cultured embryos from less than 20% to more than 94%. Without this pretreatment, most isolated embryos turned brown and died soon after culture. Through preincubation combined with partial dehydration to 18-28% moisture content, direct freezing and rapid thawing, isolated embryos were successfully cryopreserved and 31-44% emergence was achieved from postthawed embryos for the four Sabal species. |
| Keyword | Cryopreservation Embryo Culture Preincubation Sabal Zygotic Embryo |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/3846 |
| Collection | 支撑系统 |
| Recommended Citation GB/T 7714 | Wen, B,Wang, RL. Pretreatment incubation for culture and cryopreservation of Sabal embryos[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2010,102(2):237-243. |
| APA | Wen, B,&Wang, RL.(2010).Pretreatment incubation for culture and cryopreservation of Sabal embryos.PLANT CELL TISSUE AND ORGAN CULTURE,102(2),237-243. |
| MLA | Wen, B,et al."Pretreatment incubation for culture and cryopreservation of Sabal embryos".PLANT CELL TISSUE AND ORGAN CULTURE 102.2(2010):237-243. |
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