| Functional significance of asymmetrical retention of parental alleles in a hybrid pine species complex | |
| Qu, Chang; Kao, Hong-Na; Xu, Hui; Wang, Bao-Sheng; Yang, Zhi-Ling; Yang, Qi3; Liu, Gui-Feng; Wang, Xiao-Ru; Liu, Yan-Jing; Zeng, Qing-Yin | |
| 2023 | |
| Source Publication | JOURNAL OF SYSTEMATICS AND EVOLUTION
 |
| ISSN | 1674-4918 |
| Issue | xPages:- |
| Abstract | Hybrid genomes usually harbor asymmetrical parental contributions. However, it is challenging to infer the functional significance of asymmetrical retention of parental alleles in hybrid populations of conifer trees. Here we investigated the diversity in the glutathione S-transferase (GST) gene family in a hybrid pine Pinus densata and its parents (Pinus tabuliformis and Pinus yunnanensis). Plant GSTs play major roles in protecting plants against biotic and abiotic stresses. In this study, 19 orthologous groups of GST genes were identified and cloned from these three species. We examined their expression in difFerent tissues, and then purified the corresponding proteins to characterize their enzymatic activities and specificities toward difFerent substrates. We found that among the 19 GST orthologous groups, divergence in gene expression and in enzymatic activities toward difFerent substrates was prevalent. P. densata preferentially retained P. yunnanensis-like GSTs for 17 out of the 19 gene loci. We determined the first GST crystal structure from conifer species at a resolution of 2.19 A. Based on this structure, we performed site-directed mutagenesis to replace amino acid residuals in difFerent wild-types of GSTs to understand their functional impacts. Reciprocal replacement of amino acid residuals in native GSTs of P. densata and P. tabuliformis demonstrated significant changes in enzyme functions and identified key sites controlling GSTs activities. This study illustrates an approach to evaluating the functional significance of sequence variations in conifer genomes. Our study also sheds light on plausible mechanisms for controlling the selective retention of parental alleles in the P. densata genome. |
| Keyword | GLUTATHIONE-S-TRANSFERASE GENE FAMILY SPECIATION DENSATA DIVERGENCE EVOLUTION ALIGNMENT DNA |
| Subject Area | Plant Sciences |
| DOI | 10.1111/jse.12953 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000970443700001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/13447 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Northeast Forestry Univ, Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable Ut, South China Bot Garden, Guangzhou 510650, Peoples R China 3.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Menglun 666303, Yunnan, Peoples R China 4.Zhejiang A&F Univ, Coll Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou 311300, Zhejiang, Peoples R China 5.Umea Univ, Dept Ecol & Environm Sci, UPSC, SE-90187 Umea, Sweden |
| Recommended Citation GB/T 7714 | Qu, Chang,Kao, Hong-Na,Xu, Hui,et al. Functional significance of asymmetrical retention of parental alleles in a hybrid pine species complex[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2023(x):-. |
| APA | Qu, Chang.,Kao, Hong-Na.,Xu, Hui.,Wang, Bao-Sheng.,Yang, Zhi-Ling.,...&Zeng, Qing-Yin.(2023).Functional significance of asymmetrical retention of parental alleles in a hybrid pine species complex.JOURNAL OF SYSTEMATICS AND EVOLUTION(x),-. |
| MLA | Qu, Chang,et al."Functional significance of asymmetrical retention of parental alleles in a hybrid pine species complex".JOURNAL OF SYSTEMATICS AND EVOLUTION .x(2023):-. |
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| Functional significa（3062KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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