| RNA sequencing in Artemisia annua L explored the genetic and metabolic responses to hardly soluble aluminum phosphate treatment | |
| Wan, Lingyun; Huang, Qiulan; Ji, Xiaowen; Song, Lisha; Zhang, Zhanjiang; Pan, Limei; Fu, Jine; Elbaiomy, Rania G.; Eldomiaty, Ahmed S.; Rather, Shabir A.; Elashtokhy, Mohamed M. A.; Gao, Jihai5; Guan, Lingliang6; Wei, Shugen; El-Sappah, Ahmed H. | |
| 2023 | |
| Source Publication | FUNCTIONAL & INTEGRATIVE GENOMICS
 |
| ISSN | 1438-793X |
| Volume | 23Issue:2Pages:- |
| Abstract | Artemisia annua L. is a medicinal plant valued for its ability to produce artemisinin, a molecule used to treat malaria. Plant nutrients, especially phosphorus (P), can potentially influence plant biomass and secondary metabolite production. Our work aimed to explore the genetic and metabolic response of A. annua to hardly soluble aluminum phosphate (AlPO4, AlP), using soluble monopotassium phosphate (KH2PO4, KP) as a control. Liquid chromatography-mass spectrometry (LC-MS) was used to analyze artemisinin. RNA sequencing, gene ontology (GO), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were applied to analyze the differentially expressed genes (DEGs) under poor P conditions. Results showed a significant reduction in plant growth parameters, such as plant height, stem diameter, number of leaves, leaf areas, and total biomass of A. annua. Conversely, LC-MS analysis revealed a significant increase in artemisinin concentration under the AlP compared to the KP. Transcriptome analysis revealed 762 differentially expressed genes (DEGs) between the AlP and the KP. GH3, SAUR, CRE1, and PYL, all involved in plant hormone signal transduction, showed differential expression. Furthermore, despite the downregulation of HMGR in the artemisinin biosynthesis pathway, the majority of genes (ACAT, FPS, CYP71AV1, and ALDH1) were upregulated, resulting in increased artemisinin accumulation in the AlP. In addition, 12 transcription factors, including GATA and MYB, were upregulated in response to AlP, confirming their importance in regulating artemisinin biosynthesis. Overall, our findings could contribute to a better understanding the parallel transcriptional regulation of plant hormone transduction and artemisinin biosynthesis in A. annua L. in response to hardly soluble phosphorus fertilizer. |
| Keyword | TRANSCRIPTION FACTOR PHOSPHORUS ACQUISITION STARVATION RESPONSES SIGNAL-TRANSDUCTION BIOSYNTHESIS EXPRESSION ACTIVATION ROLES ACID SOIL |
| Subject Area | Genetics & Heredity |
| DOI | 10.1007/s10142-023-01067-3 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000978303000003 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/13436 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Guangxi Bot Garden Med Plants, Key Lab Guangxi High Qual Format & Utilizat Dao Di, Nanning, Peoples R China 2.Huang, Qiulan; El-Sappah, Ahmed H.] Yibin Univ, Fac Agr Forestry & Food Engn, Yibin, Peoples R China 3.Elbaiomy, Rania G.] Ahram Canadian Univ, Fac Pharm, Giza, Egypt 4.Eldomiaty, Ahmed S.; Elashtokhy, Mohamed M. A.; El-Sappah, Ahmed H.] Zagazig Univ, Fac Agr, Genet Dept, Zagazig, Egypt 5.Rather, Shabir A.] Chinese Acad Sci, Ctr Integrat Conservat, Xishuangbanna Trop Bot Garden, Mengla, Yunnan, Peoples R China 6.Chengdu Univ Tradit Chinese Med, Sch Pharm, Chengdu, Peoples R China 7.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou, Peoples R China |
| Recommended Citation GB/T 7714 | Wan, Lingyun,Huang, Qiulan,Ji, Xiaowen,et al. RNA sequencing in Artemisia annua L explored the genetic and metabolic responses to hardly soluble aluminum phosphate treatment[J]. FUNCTIONAL & INTEGRATIVE GENOMICS,2023,23(2):-. |
| APA | Wan, Lingyun.,Huang, Qiulan.,Ji, Xiaowen.,Song, Lisha.,Zhang, Zhanjiang.,...&El-Sappah, Ahmed H..(2023).RNA sequencing in Artemisia annua L explored the genetic and metabolic responses to hardly soluble aluminum phosphate treatment.FUNCTIONAL & INTEGRATIVE GENOMICS,23(2),-. |
| MLA | Wan, Lingyun,et al."RNA sequencing in Artemisia annua L explored the genetic and metabolic responses to hardly soluble aluminum phosphate treatment".FUNCTIONAL & INTEGRATIVE GENOMICS 23.2(2023):-. |
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| RNA sequencing in Ar（4025KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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