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| Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe | |
| Ji, Wei; Du, Lingyun; Zhang, Yuanfu; Liu, Guofu; Wang, Shuhao | |
| 2015 | |
| Source Publication | FOOD ANALYTICAL METHODS
 |
| ISSN | 1936-9751 |
| Volume | 8Issue:10Pages:2596-2604 |
| Abstract | An ultrasensitive fluorescence immunoassay was developed for detection of bisphenol A (BPA). Herein, thiol-modified single-strand DNA (SH-ssDNAs) and anti-BPA antibodies were simultaneously conjugated with gold nanoparticles (AuNPs), and the fluorescein isothiocyanate-labeled single-strand DNA was hybridized with SH-ssDNAs to form a dual-codified probe. In the presence of BPA, a competitive immunoreaction was conducted between BPA and BPA coating antigens immobilized on microplate for the anti-BPA antibodies of dual-codified probe. Then, in order to avoid the fluorescent signals quenched by AuNPs, restriction enzyme BamH D dagger was employed to cut the fluorescent labels from immunocomplex, resulting in an increase of fluorescence emission. Under optimized conditions, the linear range for detection of BPA is from 1.0 x 10(-2) to 1.0 x 10(3) ng/L with a low limit of detection of 3.4 x 10(-3) ng/L. This method is of good sensibility, stability, and specificity, and it can be used to detect BPA in actual milk products and its migration from packaging materials into milk. |
| Keyword | Linked-immunosorbent-assay Tandem Mass-spectrometry Human Exposure Migration Food Bpa Samples Dna Temperature Biosensor |
| Indexed By | SCI |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/9803 |
| Collection | 文献共享 |
| Recommended Citation GB/T 7714 | Ji, Wei,Du, Lingyun,Zhang, Yuanfu,et al. Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe[J]. FOOD ANALYTICAL METHODS,2015,8(10):2596-2604. |
| APA | Ji, Wei,Du, Lingyun,Zhang, Yuanfu,Liu, Guofu,&Wang, Shuhao.(2015).Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe.FOOD ANALYTICAL METHODS,8(10),2596-2604. |
| MLA | Ji, Wei,et al."Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe".FOOD ANALYTICAL METHODS 8.10(2015):2596-2604. |
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| Ultrasensitive Fluor（1055KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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