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| Transcriptomics and physiological analyses reveal co-ordinated alteration of metabolic pathways in Jatropha curcas drought tolerance | |
| Sapeta, Helena; Lourenco, Tiago; Lorenz, Stefan; Grumaz, Christian; Kirstahler, Philipp; Barros, Pedro M.; Costa, Joaquim Miguel; Sohn, Kai; Oliveira, M. Margarida | |
| 2016 | |
| Source Publication | JOURNAL OF EXPERIMENTAL BOTANY
 |
| ISSN | 0022-0957 |
| Volume | 67Issue:3Pages:845-860 |
| Abstract | Jatropha curcas, a multipurpose plant attracting a great deal of attention due to its high oil content and quality for bio-fuel, is recognized as a drought-tolerant species. However, this drought tolerance is still poorly characterized. This study aims to contribute to uncover the molecular background of this tolerance, using a combined approach of transcriptional profiling and morphophysiological characterization during a period of water-withholding (49 d) followed by rewatering (7 d). Morphophysiological measurements showed that J. curcas plants present different adaptation strategies to withstand moderate and severe drought. Therefore, RNA sequencing was performed for samples collected under moderate and severe stress followed by rewatering, for both roots and leaves. Jatropha curcas transcriptomic analysis revealed shoot- and root-specific adaptations across all investigated conditions, except under severe stress, when the dramatic transcriptomic reorganization at the root and shoot level surpassed organ specificity. These changes in gene expression were clearly shown by the down-regulation of genes involved in growth and water uptake, and up-regulation of genes related to osmotic adjustments and cellular homeostasis. However, organ-specific gene variations were also detected, such as strong up-regulation of abscisic acid synthesis in roots under moderate stress and of chlorophyll metabolism in leaves under severe stress. Functional validation further corroborated the differential expression of genes coding for enzymes involved in chlorophyll metabolism, which correlates with the metabolite content of this pathway. |
| Keyword | Chlorophyll-b Biosynthesis Arabidopsis-thaliana Gene-expression Functional-characterization Methyl Jasmonate Stomatal Closure Stress-response Water-stress a Oxygenase Plants |
| Indexed By | SCI |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/9795 |
| Collection | 文献共享 |
| Recommended Citation GB/T 7714 | Sapeta, Helena,Lourenco, Tiago,Lorenz, Stefan,et al. Transcriptomics and physiological analyses reveal co-ordinated alteration of metabolic pathways in Jatropha curcas drought tolerance[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016,67(3):845-860. |
| APA | Sapeta, Helena.,Lourenco, Tiago.,Lorenz, Stefan.,Grumaz, Christian.,Kirstahler, Philipp.,...&Oliveira, M. Margarida.(2016).Transcriptomics and physiological analyses reveal co-ordinated alteration of metabolic pathways in Jatropha curcas drought tolerance.JOURNAL OF EXPERIMENTAL BOTANY,67(3),845-860. |
| MLA | Sapeta, Helena,et al."Transcriptomics and physiological analyses reveal co-ordinated alteration of metabolic pathways in Jatropha curcas drought tolerance".JOURNAL OF EXPERIMENTAL BOTANY 67.3(2016):845-860. |
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| Transcriptomics and （2396KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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