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| Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants | |
| Wu, GH; Truksa, M; Datla, N; Vrinten, P; Bauer, J; Zank, T; Cirpus, P; Heinz, E; Qiu, X | |
| 2005 | |
| Source Publication | NATURE BIOTECHNOLOGY
 |
| ISSN | 1087-0156 |
| Volume | 23Issue:8Pages:1013-1017 |
| Abstract | Very long chain polyunsaturated fatty acids (VLCPUFAs) such as arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are valuable commodities that provide important human health benefits(1-5). We report the transgenic production of significant amounts of AA and EPA in Brassica juncea seeds via a stepwise metabolic engineering strategy. Using a series of transformations with increasing numbers of transgenes, we demonstrate the incremental production of VLCPUFAs, achieving AA levels of up to 25% and EPA levels of up to 15% of total seed fatty acids. Both fatty acids were almost exclusively found in triacylglycerols, with AA located preferentially at sn-2 and sn-3 positions and EPA distributed almost equally at all three positions. Moreover, we reconstituted the DHA biosynthetic pathway in plant seeds, demonstrating the practical feasibility of large-scale production of this important omega-3 fatty acid in oilseed crops. |
| Keyword | Brassica-napus l Docosahexaenoic Acid Functional-characterization Biosynthesis Identification Desaturase Elongation Elongases Laurate Juncea |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/9148 |
| Collection | 文献共享 |
| Recommended Citation GB/T 7714 | Wu, GH,Truksa, M,Datla, N,et al. Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants[J]. NATURE BIOTECHNOLOGY,2005,23(8):1013-1017. |
| APA | Wu, GH.,Truksa, M.,Datla, N.,Vrinten, P.,Bauer, J.,...&Qiu, X.(2005).Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants.NATURE BIOTECHNOLOGY,23(8),1013-1017. |
| MLA | Wu, GH,et al."Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants".NATURE BIOTECHNOLOGY 23.8(2005):1013-1017. |
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| Stepwise engineering（306KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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