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| TPA induces the expression of EC-SOD in human monocytic THP-1 cells: Involvement of PKC, MEK/ERK and NOX-derived ROS | |
| Makino, J; Kamiya, T; Hara, H; Adachi, T | |
| 2012 | |
| Source Publication | FREE RADICAL RESEARCH
 |
| ISSN | 1071-5762 |
| Volume | 46Issue:5Pages:637-644 |
| Abstract | Extracellular-superoxide dismutase (EC-SOD) is a major SOD isozyme mainly present in the vascular wall. EC-SOD is also observed in monocytes/macrophages, and its high expression contributes to the suppression of atherosclerosis by scavenging superoxide. The molecular mechanisms governing cell-specific expression of EC-SOD are mostly unknown, while the anti-oxidative effect of EC-SOD is well recognized. In this study, we investigated the expression of EC-SOD during 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced monocytic differentiation of THP-1 cells, which is not expressing its gene in the basal phase. We confirmed the significant induction of EC-SOD in a TPA time-dependent manner, and that induction was completely blocked by pre-treatment with GF109203X, an inhibitor of protein kinase C, U0126 and PD98059, inhibitors of mitogen-activated protein kinase kinase/extracellular-signal regulated kinase. Moreover, we determined the involvement of NADPH oxidase-derived reactive oxygen species in that induction. Overall, we considered that these results may contribute to clarify the cell-specific expression of EC-SOD. |
| Keyword | Extracellular-superoxide-dismutase Protein-kinase-c Nf-kappa-b Oxidative Stress Reactive Oxygen U937 Cells Cos7 Cells Signal-transduction Metabolic Syndrome Cobalt Chloride |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/9128 |
| Collection | 文献共享 |
| Recommended Citation GB/T 7714 | Makino, J,Kamiya, T,Hara, H,et al. TPA induces the expression of EC-SOD in human monocytic THP-1 cells: Involvement of PKC, MEK/ERK and NOX-derived ROS[J]. FREE RADICAL RESEARCH,2012,46(5):637-644. |
| APA | Makino, J,Kamiya, T,Hara, H,&Adachi, T.(2012).TPA induces the expression of EC-SOD in human monocytic THP-1 cells: Involvement of PKC, MEK/ERK and NOX-derived ROS.FREE RADICAL RESEARCH,46(5),637-644. |
| MLA | Makino, J,et al."TPA induces the expression of EC-SOD in human monocytic THP-1 cells: Involvement of PKC, MEK/ERK and NOX-derived ROS".FREE RADICAL RESEARCH 46.5(2012):637-644. |
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