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| Transgenic Arabidopsis Flowers Overexpressing Acyl-CoA-Binding Protein ACBP6 are Freezing Tolerant | |
| Liao, P; Chen, QF; Chye, ML | |
| 2014 | |
| Source Publication | PLANT AND CELL PHYSIOLOGY
 |
| ISSN | 0032-0781 |
| Volume | 55Issue:6Pages:1055-1071 |
| Abstract | Low temperature stress adversely affects plant growth. It has been shown that the overexpression of ACYL-COENZYME A-BINDING PROTEIN6 (ACBP6) resulted in enhanced freezing tolerance in seedlings and rosettes accompanied by a decrease in phosphatidylcholine (PC), an increase in phosphatidic acid (PA) and an up-regulation of PHOSPHOLIPASE D delta(PLD delta) in the absence of COLD-RESPONSIVE (COR)-related gene induction. Unlike rosettes, ACBP6-overexpressor (OE) flowers showed elevations in PC and monogalactosyldiacylglycerol (MGDG) accompanied by a decline in PA. The increase in PC species corresponded to a decline in specific PAs. To better understand such differences, the expression of PC-, MGDG-, proline-, ABA- and COR-related genes, and their transcription factors [C-repeat binding factors (CBFs), INDUCER OF CBF EXPRESSION1 (ICE1) and MYB15] was analyzed by quantitative real-time PCR (qRT-PCR). ACBP6-conferred freezing-tolerant flowers showed induction of COR-related genes, CBF genes and ICE1, PC-related genes (PLD delta, CK, CK-LIKE1, CK-LIKE2, CCT1, CCT2, LPCAT1, PLA2 alpha, PAT-PLA-II beta, PAT-PLA-III alpha, PAT-PLA-III delta and PLD zeta 2), MGDG-related genes (MGD genes and SFR2) and ABA-responsive genes. In contrast, ACBP6-conferred freezing-tolerant rosettes were down-regulated in COR-related genes, CBF1, PC-related genes (PEAMT1, PEAMT2, PEAMT3, CK1, CCT1, CCT2, PLA2 alpha, PAT-PLA-III delta and PLD zeta 2), MGDG-related genes (MGD2, MGD3 and SFR2) and some ABA-responsive genes including KIN1 and KIN2. These results suggest that the mechanism in ACBP6-conferred freezing tolerance varies in different organs. |
| Keyword | Plant Cold-acclimation Gene-expression Low-temperature Transcriptional Activators Plasma-membrane Abscisic-acid Salt Stress Thaliana Biosynthesis Drought |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/9114 |
| Collection | 文献共享 |
| Recommended Citation GB/T 7714 | Liao, P,Chen, QF,Chye, ML. Transgenic Arabidopsis Flowers Overexpressing Acyl-CoA-Binding Protein ACBP6 are Freezing Tolerant[J]. PLANT AND CELL PHYSIOLOGY,2014,55(6):1055-1071. |
| APA | Liao, P,Chen, QF,&Chye, ML.(2014).Transgenic Arabidopsis Flowers Overexpressing Acyl-CoA-Binding Protein ACBP6 are Freezing Tolerant.PLANT AND CELL PHYSIOLOGY,55(6),1055-1071. |
| MLA | Liao, P,et al."Transgenic Arabidopsis Flowers Overexpressing Acyl-CoA-Binding Protein ACBP6 are Freezing Tolerant".PLANT AND CELL PHYSIOLOGY 55.6(2014):1055-1071. |
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| Transgenic Arabidops（3098KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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