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| Fluorescence Correlation Spectroscopy, Raster Image Correlation Spectroscopy, and Number and Brightness on a Commercial Confocal Laser Scanning Microscope with Analog Detectors (Nikon C1) | |
| Moens, P. D. J. | |
| 2011 | |
| Source Publication | Microscopy Research and Technique
 |
| ISSN | 1059-910X |
| Volume | 74Issue:4Pages:377-388 |
| Abstract | Fluorescence correlation spectroscopy (FCS) was developed in 1972 by Magde, Elson and Webb. Photon counting detectors and avalanche photodiodes have become standards in FCS to the point that there is a widespread belief that these detectors are essential to perform FCS experiments, despite the fact that FCS was developed using analog detectors. Spatial and temporal intensity fluctuation correlations using analog detection on a commercial Olympus Fluoview 300 microscope have been reported by Brown et al. (2008). However, each analog instrument has its own idiosyncrasies that need to be understood before using the instrument for FCS. In this work, we explore the capabilities of the Nikon C1, a low-cost confocal microscope, to obtain single point FCS, Raster-scan image correlation spectroscopy (RICS), and Number and Brightness data both in solution and incorporated into the membrane of giant unilamellar vesicles. We show that it is possible to obtain dynamic information about fluorescent molecules from single point FCS, RICS, and Number and Brightness using the Nikon C1. We highlighted the fact that care should be taken in selecting the acquisition parameters to avoid possible artifacts due to the detector noise. However, due to relatively large errors in determining the distribution of digital levels for a given microscope setting, the system is probably only adequate for determining relative brightness within the same image. Microsc. Res. Tech. 74:377-388, 2011. (C) 2010 Wiley-Liss, Inc. |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8968 |
| Collection | 公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| Recommended Citation GB/T 7714 | Moens, P. D. J.. Fluorescence Correlation Spectroscopy, Raster Image Correlation Spectroscopy, and Number and Brightness on a Commercial Confocal Laser Scanning Microscope with Analog Detectors (Nikon C1)[J]. Microscopy Research and Technique,2011,74(4):377-388. |
| APA | Moens, P. D. J..(2011).Fluorescence Correlation Spectroscopy, Raster Image Correlation Spectroscopy, and Number and Brightness on a Commercial Confocal Laser Scanning Microscope with Analog Detectors (Nikon C1).Microscopy Research and Technique,74(4),377-388. |
| MLA | Moens, P. D. J.."Fluorescence Correlation Spectroscopy, Raster Image Correlation Spectroscopy, and Number and Brightness on a Commercial Confocal Laser Scanning Microscope with Analog Detectors (Nikon C1)".Microscopy Research and Technique 74.4(2011):377-388. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Fluorescence and con（760KB） | 开放获取 | CC BY-NC-SA | View Download | |||
| Fluorescence Correla（909KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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