Institutional Repository of Xishuangbanna Tropical Botanical Garden
| Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants | |
| Stuurman, N. | |
| 2000 | |
| Source Publication | Molecular Plant-Microbe Interactions
 |
| ISSN | 0894-0282 |
| Volume | 13Issue:11Pages:1163-1169 |
| Abstract | We developed two sets of broad-host-range vectors that drive expression of the green fluorescent protein (GFP) or color variants thereof (henceforth collectively called autofluorescent proteins [AFPs]) from the lac promoter, These two sets are based on different replicons that are maintained in a stable fashion in Escherichia coli and rhizobia. Using specific filter sets or a dedicated confocal laser scanning microscope setup in which emitted light is split into its color components through a prism, we were able to unambiguously identify bacteria expressing enhanced cyan fluorescent protein (ECFP) or enhanced yellow fluorescent protein (EYFP) in mixtures of the two. Clearly, these vectors will be valuable tools for competition, cohabitation, and rescue studies and will also allow the visualization of interactions between genetically marked bacteria in vivo. Here, we used these vectors to visualize the interaction between rhizobia and plants. Specifically, we found that progeny from different rhizobia can be found in the same nodule or even in the same infection thread, We also visualized movements of bacteroids within plant nodule cells. |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8937 |
| Collection | 公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| Recommended Citation GB/T 7714 | Stuurman, N.. Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants[J]. Molecular Plant-Microbe Interactions,2000,13(11):1163-1169. |
| APA | Stuurman, N..(2000).Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants.Molecular Plant-Microbe Interactions,13(11),1163-1169. |
| MLA | Stuurman, N.."Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants".Molecular Plant-Microbe Interactions 13.11(2000):1163-1169. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Use of green fluores（254KB） | 开放获取 | CC BY-NC-SA | View Download | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment