Institutional Repository of Xishuangbanna Tropical Botanical Garden
| Identifying biological landmarks using a novel cell measuring image analysis tool: Cell-o-Tape | |
| French, A. P. | |
| 2012 | |
| Source Publication | Plant Methods
 |
| ISSN | 1746-4811 |
| Volume | 8Issue:xPages:7 |
| Abstract | Background: The ability to quantify the geometry of plant organs at the cellular scale can provide novel insights into their structural organization. Hitherto manual methods of measurement provide only very low throughput and subjective solutions, and often quantitative measurements are neglected in favour of a simple cell count. Results: We present a tool to count and measure individual neighbouring cells along a defined file in confocal laser scanning microscope images. The tool allows the user to extract this generic information in a flexible and intuitive manner, and builds on the raw data to detect a significant change in cell length along the file. This facility can be used, for example, to provide an estimate of the position of transition into the elongation zone of an Arabidopsis root, traditionally a location sensitive to the subjectivity of the experimenter. Conclusions: Cell-o-tape is shown to locate cell walls with a high degree of accuracy and estimate the location of the transition feature point in good agreement with human experts. The tool is an open source ImageJ/Fiji macro and is available online. |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8928 |
| Collection | 公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| Recommended Citation GB/T 7714 | French, A. P.. Identifying biological landmarks using a novel cell measuring image analysis tool: Cell-o-Tape[J]. Plant Methods,2012,8(x):7. |
| APA | French, A. P..(2012).Identifying biological landmarks using a novel cell measuring image analysis tool: Cell-o-Tape.Plant Methods,8(x),7. |
| MLA | French, A. P.."Identifying biological landmarks using a novel cell measuring image analysis tool: Cell-o-Tape".Plant Methods 8.x(2012):7. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Identifying biologic（3086KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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