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| Selective detection of superoxide anion radicals generated from macrophages by using a novel fluorescent probe | |
| Gao, J. J. | |
| 2007 | |
| Source Publication | Febs Journal
 |
| ISSN | 1742-464X |
| Volume | 274Issue:7Pages:1725-1733 |
| Abstract | Quantitation of superoxide radical (O-2(-center dot)) production at the site of radical generation remains challenging. A simple method to detect nanomolar to micromolar levels of superoxide radical in aqueous solution has been developed and optimized. This method is based on the efficient trapping of O-2(-center dot) using a novel fluorescent probe (2-chloro-1,3-dibenzothiazolinecyclohexene), coupled with a spectra character-signaling increase event. A high-specificity and high-sensitivity fluorescent probe was synthesized in-house and used to image O-2(-center dot) in living cells. Better selectivity for O-2(-center dot) over competing cellular reactive oxygen species and some biological compounds illustrates the advantages of our method. Under optimal conditions, the linear calibration range for superoxide anion radicals was 5.03 x 10(-9)-3.33 x 10(-6) M. The detection limit was 1.68 x 10(-9) M. Fluorescence images of probe-stained macrophages stimulated with 4 beta-phorbol 12-myristate 13-acetate were obtained successfully using a confocal laser scanning microscope. |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8887 |
| Collection | 公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| Recommended Citation GB/T 7714 | Gao, J. J.. Selective detection of superoxide anion radicals generated from macrophages by using a novel fluorescent probe[J]. Febs Journal,2007,274(7):1725-1733. |
| APA | Gao, J. J..(2007).Selective detection of superoxide anion radicals generated from macrophages by using a novel fluorescent probe.Febs Journal,274(7),1725-1733. |
| MLA | Gao, J. J.."Selective detection of superoxide anion radicals generated from macrophages by using a novel fluorescent probe".Febs Journal 274.7(2007):1725-1733. |
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| File Name/Size | DocType | Version | Access | License | ||
| Selective detection （727KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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