Volume Reconstruction of Large Tissue Specimens From Serial Physical Sections Using Confocal Microscopy and Correction of Cutting Deformations by Elastic Registration

XTBG OpenIR > 公共技术服务中心主要仪器相关文献 > 激光共聚焦显微镜

	Volume Reconstruction of Large Tissue Specimens From Serial Physical Sections Using Confocal Microscopy and Correction of Cutting Deformations by Elastic Registration
	Capek, M.
	2009
Source Publication	Microscopy Research and Technique
ISSN	1059-910X
Volume	72 Issue:2 Pages:110-119
Abstract	A set of methods leading to volume reconstruction of biological specimens larger than the field of view of a confocal laser scanning microscope (CLSM) is presented. Large tissue specimens are cut into thin physical slices and volume data sets are captured from all studied physical slices by CLSM. Overlapping spatial tiles of the same physical slice are stitched in horizontal direction. Image volumes of successive physical slices are linked in axial direction by applying an elastic registration algorithm to compensate for deformations because of cutting the specimen. We present a method enabling us to keep true object morphology using a priori information about the shape and size of the specimen, available from images of the cutting planes captured by a USB light microscope immediately before cutting the specimen by a microtome. The errors introduced by elastic registration are evaluated using a stereological point counting method and the Procrustes distance. Finally, the images are enhanced to compensate for the effect of the light attenuation with depth and visualized by a hardware accelerated volume rendering. Algorithmic steps of the reconstruction, namely elastic registration, object morphology preservation, image enhancement, and volume visualization, are implemented in a new Rapid3D software package. Because confocal microscopes get more and more frequently used in scientific laboratories, the described volume reconstruction may become an easy-to-apply tool to study large biological objects, tissues, and organs in histology, embryology, evolution biology, and developmental biology. In this work, we demonstrate the reconstruction using a postcranial part of a 17-day-old laboratory Wistar rat embryo. Microsc. Res. Tech. 72:110-119, 2009. (C) 2008 Wiley-Liss. Inc.
Language	英语
Document Type	期刊论文
Identifier	https://ir.xtbg.ac.cn/handle/353005/8874
Collection	公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
Recommended Citation GB/T 7714	Capek, M.. Volume Reconstruction of Large Tissue Specimens From Serial Physical Sections Using Confocal Microscopy and Correction of Cutting Deformations by Elastic Registration[J]. Microscopy Research and Technique,2009,72(2):110-119.
APA	Capek, M..(2009).Volume Reconstruction of Large Tissue Specimens From Serial Physical Sections Using Confocal Microscopy and Correction of Cutting Deformations by Elastic Registration.Microscopy Research and Technique,72(2),110-119.
MLA	Capek, M.."Volume Reconstruction of Large Tissue Specimens From Serial Physical Sections Using Confocal Microscopy and Correction of Cutting Deformations by Elastic Registration".Microscopy Research and Technique 72.2(2009):110-119.

Files in This Item:		Download All
File Name/Size	DocType	Version	Access	License
Volume Reconstructio（568KB）			开放获取	CC BY-NC-SA	View Download

File name:	Volume Reconstruction of Large Tissue Specimens From Serial Physical Sections Using Confocal Microscopy and Correction of Cutting Deformations by Elastic Registration.pdf
Format:	Adobe PDF

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Institution:
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh

中国科学院西双版纳热带植物园机构知识库