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| Cortactin associates with the cell-cell junction protein ZO-1 in both Drosophila and mouse | |
| Katsube, T. | |
| 1998 | |
| Source Publication | Journal of Biological Chemistry
 |
| ISSN | 0021-9258 |
| Volume | 273Issue:45Pages:29672-29677 |
| Abstract | Cortactin is an actin filament-binding protein localizing at cortical regions of cells and a prominent substrate for Src family protein-tyrosine kinases in response to multiple extracellular stimuli. Human cortactin has been identified as a protein product of a putative oncogene, EMS1. In this report, we describe the identification of a Drosophila homolog of cortactin as a molecule that interacts with Drosophila ZO-1 using yeast two-hybrid screening. Drosophila cortactin is a 559-amino acid protein highly expressed in embryos, larvae, and pupae but relatively underexpressed in adult flies. Deletion and substitution mutant analyses revealed that the SH3 domain of Drosophila cortactin binds to a PXXP motif in the proline-rich domain of Drosophila ZO-1. Colocalization of these proteins at cell-cell junction sites was evident under a confocal laser-scanning microscope. In vivo association was confirmed by coimmunoprecipitation of cortactin and ZO-1 from Drosophila embryo lysates. We also demonstrate an association for each of the murine homologs by immunoprecipitation analyses of mouse tissue lysates. Our previous work has demonstrated the involvement of ZO-1 in a signaling pathway that regulates expression of the emc gene in Drosophila. The potential roles of the cortactin.ZO-1 complex in cell adhesion and cell signaling are discussed. |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8861 |
| Collection | 公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| Recommended Citation GB/T 7714 | Katsube, T.. Cortactin associates with the cell-cell junction protein ZO-1 in both Drosophila and mouse[J]. Journal of Biological Chemistry,1998,273(45):29672-29677. |
| APA | Katsube, T..(1998).Cortactin associates with the cell-cell junction protein ZO-1 in both Drosophila and mouse.Journal of Biological Chemistry,273(45),29672-29677. |
| MLA | Katsube, T.."Cortactin associates with the cell-cell junction protein ZO-1 in both Drosophila and mouse".Journal of Biological Chemistry 273.45(1998):29672-29677. |
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| Cortactin associates（1190KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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