Institutional Repository of Xishuangbanna Tropical Botanical Garden
| Analysis of fluorescence from algae fossils of the Neoproterozoic Doushantuo Formation of China by confocal laser scanning microscope | |
| Chi, H. M. | |
| 2006 | |
| Source Publication | Microscopy Research and Technique
 |
| ISSN | 1059-910X |
| Volume | 69Issue:4Pages:253-259 |
| Abstract | Chinese algae fossils can provide unique information about the evolution of the early life. Thin sections of Neoproterozoic algae fossils, from Guizhou, China, were studied by confocal laser scanning microscopy, and algae fossils were fluorescenced at different wavelengths when excited by laser light of 488 nm, 476 nm, and 568 run wavelength. When illuminated by 488 nm laser light, images of the algae fossils were sharper and better defined than when illuminated by 476 nm and 568 nm laser light. The algae fossils fluoresce at a wide range of emission wavelengths. The three-dimensional images of the fluorescent algae fossils were compared with the transmission images taken by light microscope. We found that the fluorescence image of the confocal laser scanning microscope in a single optical section could pass for the transmission image taken by a light microscope. We collected images at different sample depths and made a three-dimensional reconstruction of the algae fossils. And on the basis of the reconstruction of the three-dimensional fluorescent images, we conclude that the two algae fossils in our present study are red algae. |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8852 |
| Collection | 公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| Recommended Citation GB/T 7714 | Chi, H. M.. Analysis of fluorescence from algae fossils of the Neoproterozoic Doushantuo Formation of China by confocal laser scanning microscope[J]. Microscopy Research and Technique,2006,69(4):253-259. |
| APA | Chi, H. M..(2006).Analysis of fluorescence from algae fossils of the Neoproterozoic Doushantuo Formation of China by confocal laser scanning microscope.Microscopy Research and Technique,69(4),253-259. |
| MLA | Chi, H. M.."Analysis of fluorescence from algae fossils of the Neoproterozoic Doushantuo Formation of China by confocal laser scanning microscope".Microscopy Research and Technique 69.4(2006):253-259. |
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| Analysis of fluoresc（683KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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