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Use of the confocal laser scanning microscope in studies on the developmental biology of marine crustaceans | |
Buttino, I. | |
2003 | |
Source Publication | Microscopy Research and Technique
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ISSN | 1059-910X |
Volume | 60Issue:4Pages:458-464 |
Abstract | Confocal Laser Scanning Microscope techniques have been applied to study the developmental biology of marine copepods and decapod larvae. The lipophylic probes Dil and DiOC(6) were used to study both the external and internal morphology of these crustaceans, whereas the same DiOC(6) and the specific nuclear probe Hoechst 33342 were used to study embryonic development of copepods in vivo. To distinguish viable from non-viable copepod embryos, the vital dye dichlorodihydrofluorescein diacetate (H(2)DCFDA) was used. Major advantages and difficulties in the use of these non-invasive techniques in studies of the reproductive biology of marine crustaceans are discussed. (C) 2003 Wiley-Liss, Inc. |
Language | 英语 |
Document Type | 期刊论文 |
Identifier | https://ir.xtbg.ac.cn/handle/353005/8809 |
Collection | 公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
Recommended Citation GB/T 7714 | Buttino, I.. Use of the confocal laser scanning microscope in studies on the developmental biology of marine crustaceans[J]. Microscopy Research and Technique,2003,60(4):458-464. |
APA | Buttino, I..(2003).Use of the confocal laser scanning microscope in studies on the developmental biology of marine crustaceans.Microscopy Research and Technique,60(4),458-464. |
MLA | Buttino, I.."Use of the confocal laser scanning microscope in studies on the developmental biology of marine crustaceans".Microscopy Research and Technique 60.4(2003):458-464. |
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