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| Bacterial inactivation in water, DNA strand breaking, and membrane damage induced by ultraviolet-assisted titanium dioxide photocatalysis | |
| Kim, S. | |
| 2013 | |
| Source Publication | Water Research
 |
| ISSN | 0043-1354 |
| Volume | 47Issue:13Pages:4403-4411 |
| Abstract | The effects of UV-assisted TiO2-photocatalytic oxidation (PCO) inactivation of pathogenic bacteria (Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium) in a liquid culture using different domains of UV irradiation (A, B and C) were evaluated. Structural changes in super-coiled plasmid DNA (pUC19) and genomic DNA of E. coli were observed using gel electrophoresis to demonstrate the photodynamic DNA strand breaking activity of UV-assisted TiO2-PCO. Membrane damage in bacterial cells was observed using both a scanning electron microscope (SEM) and a confocal laser scanning microscope (CLSM). Both UVC-TiO2-PCO and UVC alone resulted in an earlier bactericidal phase (initial counts of approximately 6 log CFU/mL) in 60 s and 90 s, respectively, in liquid culture. UVC-TiO2-PCO treatment for 6 min converted all plasmid DNA to the linear form; however, under UVC irradiation alone, super-coiled DNA remained. Prolonged UVC-TiO2-PCO treatment resulted in structural changes in genomic DNA from E. coli. SEM observations revealed that bacteria suffered severe visible cell damage after UVC-TiO2-PCO treatment for 30-60 mm. S. typhimurium cells showed visible damage after 30 min, which was confirmed using CLSM. All treated cells were stained red using propidium iodide under a fluorescent light. (C) 2013 Elsevier Ltd. All rights reserved. |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8760 |
| Collection | 公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| Recommended Citation GB/T 7714 | Kim, S.. Bacterial inactivation in water, DNA strand breaking, and membrane damage induced by ultraviolet-assisted titanium dioxide photocatalysis[J]. Water Research,2013,47(13):4403-4411. |
| APA | Kim, S..(2013).Bacterial inactivation in water, DNA strand breaking, and membrane damage induced by ultraviolet-assisted titanium dioxide photocatalysis.Water Research,47(13),4403-4411. |
| MLA | Kim, S.."Bacterial inactivation in water, DNA strand breaking, and membrane damage induced by ultraviolet-assisted titanium dioxide photocatalysis".Water Research 47.13(2013):4403-4411. |
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| File Name/Size | DocType | Version | Access | License | ||
| Bacterial inactivati（1644KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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