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| Methods for compensation of the light attenuation with depth of images captured by a confocal microscope | |
| Capek, M. | |
| 2006 | |
| Source Publication | Microscopy Research and Technique
 |
| ISSN | 1059-910X |
| Volume | 69Issue:8Pages:624-635 |
| Abstract | A confocal laser scanning microscope (CLSM) enables us to capture images from a biological specimen in different depths and obtain a series of precisely registered fluorescent images. However, images captured from deep layers of the specimen may be darker than images from the topmost layers because of light loss distortions. This effect causes difficulties in subsequent analysis of biological objects. We propose a solution using two approaches: either an online method working already during image acquisition or an offline method assisting as a postprocessing step. In the online method, the gain value of a photomultiplier tube of a CLSM is controlled according to the difference of mean image intensities between the reference and currently acquired image. The offline method consists of two stages. In the first stage, a standard histogram maintaining relative frequencies of gray levels and improving brightness and contrast is created from all images in the series. In the second stage, individual image histograms are warped according to this standard histogram. The methods were tested on real confocal image data captured from human placenta and rat skeletal muscle specimens. It was shown that both approaches diminish the light attenuation in images captured from deep layers of the specimen. |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8756 |
| Collection | 公共技术服务中心主要仪器相关文献_激光共聚焦显微镜 |
| Recommended Citation GB/T 7714 | Capek, M.. Methods for compensation of the light attenuation with depth of images captured by a confocal microscope[J]. Microscopy Research and Technique,2006,69(8):624-635. |
| APA | Capek, M..(2006).Methods for compensation of the light attenuation with depth of images captured by a confocal microscope.Microscopy Research and Technique,69(8),624-635. |
| MLA | Capek, M.."Methods for compensation of the light attenuation with depth of images captured by a confocal microscope".Microscopy Research and Technique 69.8(2006):624-635. |
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| Methods for compensa（1001KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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