| Isolation and characterization of an ubiquitin extension protein gene (JcUEP) promoter from Jatropha curcas | |
Tao, YB; He, LL; Niu, LJ; Xu, ZF
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| 2015 | |
| Source Publication | PLANTA
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| ISSN | 0032-0935 |
| Volume | 241Issue:4Pages:823-836 |
| Abstract | The JcUEP promoter is active constitutively in the bio-fuel plant Jatropha curcas , and is an alternative to the widely used CaMV35S promoter for driving constitutive overexpression of transgenes in Jatropha. Well-characterized promoters are required for transgenic breeding of Jatropha curcas, a biofuel feedstock with great potential for production of bio-diesel and bio-jet fuel. In this study, an ubiquitin extension protein gene from Jatropha, designated JcUEP, was identified to be ubiquitously expressed. Thus, we isolated a 1.2 kb fragment of the 5' flanking region of JcUEP and evaluated its activity as a constitutive promoter in Arabidopsis and Jatropha using the beta-glucuronidase (GUS) reporter gene. As expected, histochemical GUS assay showed that the JcUEP promoter was active in all Arabidopsis and Jatropha tissues tested. We also compared the activity of the JcUEP promoter with that of the cauliflower mosaic virus 35S (CaMV35S) promoter, a well-characterized constitutive promoter conferring strong transgene expression in dicot species, in various tissues of Jatropha. In a fluorometric GUS assay, the two promoters showed similar activities in stems, mature leaves and female flowers; while the CaMV35S promoter was more effective than the JcUEP promoter in other tissues, especially young leaves and inflorescences. In addition, the JcUEP promoter retained its activity under stress conditions in low temperature, high salt, dehydration and exogenous ABA treatments. These results suggest that the plant-derived JcUEP promoter could be an alternative to the CaMV35S promoter for driving constitutive overexpression of transgenes in Jatropha and other plants. |
| Keyword | Physic Nut Constitutive Promoter Ubiquitin Transgenic Stress Camv35s |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8675 |
| Collection | 能源植物分子育种研究组 |
| Corresponding Author | Xu, ZF |
| Recommended Citation GB/T 7714 | Tao, YB,He, LL,Niu, LJ,et al. Isolation and characterization of an ubiquitin extension protein gene (JcUEP) promoter from Jatropha curcas[J]. PLANTA,2015,241(4):823-836. |
| APA | Tao, YB,He, LL,Niu, LJ,&Xu, ZF.(2015).Isolation and characterization of an ubiquitin extension protein gene (JcUEP) promoter from Jatropha curcas.PLANTA,241(4),823-836. |
| MLA | Tao, YB,et al."Isolation and characterization of an ubiquitin extension protein gene (JcUEP) promoter from Jatropha curcas".PLANTA 241.4(2015):823-836. |
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