Institutional Repository of Xishuangbanna Tropical Botanical Garden
| Suitable transfection methods for single particle tracing in plant suspension cells | |
| Gohring, J. | |
| 2014 | |
| Source Publication | Plant Methods
 |
| ISSN | 1746-4811 |
| Volume | 10Pages:7 |
| Abstract | Background A multitude of different imaging systems are already available to image genetically altered RNA species; however, only a few of these techniques are actually suitable to visualize endogenous RNA. One possibility is to use fluorescently-labelled and hybridization-sensitive probes. In order to yield more information about the exact localization and movement of a single RNA molecule, it is necessary to image such probes with highly sensitive microscope setups. More challenges arise if such experiments are conducted in plant cells due to their high autofluorescence and demanding transfection procedures. Results Here, we report in planta imaging of single RNA molecules using fluorescently labeled molecular beacons. We tested three different transfection protocols in order to identify optimal conditions for transfection of fluorescent DNA probes and their subsequent detection at the single molecule level. Conclusions We found that an optimized heat shock protocol provided a vastly improved transfection method for small DNA molecules which were used for subsequent single RNA molecule detection in living plant suspension cells. |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8590 |
| Collection | 公共技术服务中心主要仪器相关文献_植物活体成像系统 |
| Recommended Citation GB/T 7714 | Gohring, J.. Suitable transfection methods for single particle tracing in plant suspension cells[J]. Plant Methods,2014,10:7. |
| APA | Gohring, J..(2014).Suitable transfection methods for single particle tracing in plant suspension cells.Plant Methods,10,7. |
| MLA | Gohring, J.."Suitable transfection methods for single particle tracing in plant suspension cells".Plant Methods 10(2014):7. |
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| Suitable transfectio（1307KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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