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| Solubilization, Fractionation, and Electrophoretic Characterization of Inca Peanut (Plukenetia volubilis L.) Proteins | |
| Sathe, S. K. | |
| 2012 | |
| Source Publication | Plant Foods for Human Nutrition
 |
| ISSN | 0921-9668 |
| Volume | 67Issue:3Pages:247-255 |
| Abstract | Effects of different solvents, ionic strength, and pH on Inca peanut seed protein solubility were assessed by quantitatively analyzing solubilized proteins using Lowry and Bradford methods. Soluble proteins were fractionated using Osborne procedure and the polypeptide composition of solubilized proteins was determined by one dimensional 25 % monomer acrylamide linear gradient SDS-PAGE. Osborne protein fractions were analyzed by the 2D gel electrophoresis. Total seed proteins were efficiently solubilized by 2 M NaCl among the tested solvents. The soluble seed proteins registered a minimum solubility at pH similar to 4.0. Osborne protein fractions, albumins, globulins, prolamins, and glutelins accounted for 43.7, 27.3, 3.0, and 31.9 %, respectively, of the total aqueous soluble proteins. Soluble seed flour proteins are mainly composed of polypeptides in the MW range of 6-70 kDa of which the predominant polypeptides were in the 20-40 kDa range. Prolamin fraction was mainly composed of four polypeptides (MW < 15 kDa). Glycoprotein staining indicated 32-35 and < 14 kDa peptides to be positive. |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8328 |
| Collection | 热带植物资源可持续利用重点实验室特色文献_星油藤相关文献 |
| Recommended Citation GB/T 7714 | Sathe, S. K.. Solubilization, Fractionation, and Electrophoretic Characterization of Inca Peanut (Plukenetia volubilis L.) Proteins[J]. Plant Foods for Human Nutrition,2012,67(3):247-255. |
| APA | Sathe, S. K..(2012).Solubilization, Fractionation, and Electrophoretic Characterization of Inca Peanut (Plukenetia volubilis L.) Proteins.Plant Foods for Human Nutrition,67(3),247-255. |
| MLA | Sathe, S. K.."Solubilization, Fractionation, and Electrophoretic Characterization of Inca Peanut (Plukenetia volubilis L.) Proteins".Plant Foods for Human Nutrition 67.3(2012):247-255. |
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| File Name/Size | DocType | Version | Access | License | ||
| Solubilization, Frac（981KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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