Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas
Sujatha, M.
1996
Source PublicationPlant Cell Tissue and Organ Culture
ISSN0167-6857
Volume44Issue:2Pages:135-141
AbstractTechniques for the regeneration of Jatropha curcas L. from various explants have been developed. Regeneration from hypocotyl, petiole and leaf explants was evaluated on a range of concentrations of zeatin, kinetin and N-6 benzyladenine (BA) either singly or in combination with indole-3-butyric acid (IBA). Higher regeneration from hypocotyl and petiole explants was obtained on BA with IBA than on zeatin- or kinetin- supplemented media. Leaf discs from the third expanding leaf exhibited higher regeneration potential than those from the fourth leaf. Independent of the explant type, direct adventitious shoot bud induction was recorded highest on MS medium with 2.22 mu M BA and 4.9 mu M IBA. Although the same BA concentration but with reduced IBA concentration (0.49 mu M) proved effective in callus mediated regeneration from hypocotyl and leaf explants, the petioles required lower concentrations of the two growth regulators (0.44 mu M BA and 0.49 mu M IBA). Regenerated shoots could be rooted on growth regulator-free gelled full-strength MS medium. Following simple hardening procedures, the in vitro-raised plants could be transferred to soil and grown to maturity in the field.
Document Type期刊论文
Identifierhttps://ir.xtbg.ac.cn/handle/353005/8075
Collection热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
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GB/T 7714
Sujatha, M.. Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas[J]. Plant Cell Tissue and Organ Culture,1996,44(2):135-141.
APA Sujatha, M..(1996).Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas.Plant Cell Tissue and Organ Culture,44(2),135-141.
MLA Sujatha, M.."Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas".Plant Cell Tissue and Organ Culture 44.2(1996):135-141.
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