Age and orientation of the cotyledonary leaf explants determine the efficiency of de novo plant regeneration and Agrobacterium tumefaciens-mediated transformation in Jatropha curcas L

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	Age and orientation of the cotyledonary leaf explants determine the efficiency of de novo plant regeneration and Agrobacterium tumefaciens-mediated transformation in Jatropha curcas L
	Mazumdar, P.
	2010
Source Publication	South African Journal of Botany
ISSN	0254-6299
Volume	76 Issue:2 Pages:337-344
Abstract	Effects of age and orientation of the explant on callus induction and de novo shoot regeneration from cotyledonary leaf segments of Jatropha curcas were studied. The callus induction and shoot regeneration capacity of cotyledonary leaf segments were found significantly related to the age of the explants and their orientation in culture medium. The youngest explant, derived from the cotyledonary leaf of germinated seed induced the highest regeneration response as compared to one- and two-week-old explants. A gradient response with age of the explant was observed in percentage of callus induction, shoot regeneration from callus and the number of shoots per regenerating callus. The explants cultured with their abaxial side in medium showed significantly higher regeneration response. The youngest explant was found to be most amenable to Agrobacterium-mediated transformation as compared to older explants. The fact that callus induced from the edges of the explant followed by de novo shoot induction, and strong transient gus expression observed in the edges of the explant are significant for routine Agrobacterium-mediated transformation and generation of stable transgenic plants in J. curcas. (C) 2010 SAAB. Published by Elsevier B.V. All rights reserved.
Document Type	期刊论文
Identifier	https://ir.xtbg.ac.cn/handle/353005/8050
Collection	热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
Recommended Citation GB/T 7714	Mazumdar, P.. Age and orientation of the cotyledonary leaf explants determine the efficiency of de novo plant regeneration and Agrobacterium tumefaciens-mediated transformation in Jatropha curcas L[J]. South African Journal of Botany,2010,76(2):337-344.
APA	Mazumdar, P..(2010).Age and orientation of the cotyledonary leaf explants determine the efficiency of de novo plant regeneration and Agrobacterium tumefaciens-mediated transformation in Jatropha curcas L.South African Journal of Botany,76(2),337-344.
MLA	Mazumdar, P.."Age and orientation of the cotyledonary leaf explants determine the efficiency of de novo plant regeneration and Agrobacterium tumefaciens-mediated transformation in Jatropha curcas L".South African Journal of Botany 76.2(2010):337-344.

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