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| Changes in morphology and biochemical indices in browning callus derived from Jatropha curcas hypocotyls | |
| He, Y. | |
| 2009 | |
| Source Publication | Plant Cell Tissue and Organ Culture
 |
| ISSN | 0167-6857 |
| Volume | 98Issue:1Pages:42325 |
| Abstract | Callus browning is a typical feature of callus cultures derived from the hypocotyl of Jatropha curcas. Brown callus results in decreased regenerative ability, poor growth and even death. In this study, we investigated the effect of browning on callus morphology and biochemical indices. Light microscopy and scanning electron microscopy showed striking differences in callus morphology. During browning, chlorophylls and carotenoids concentrations decreased steadily. Polyphenol oxidase (PPO) and peroxidase (POD) enzymatic activities patterns were similar during callus culture with a higher activity level at week 3 compared to week 2 or later weeks. Grey relation degree analysis indicated that PPO played a more important role than POD in enzymatic callus browning. Polyacrylamide gel electrophoresis results showed differences between browning and non-browning callus. Gas chromatography-mass spectrometry results showed that saturated and unsaturated fatty acid quantities differed significantly but there was little difference in fatty acid composition between non-browning and browning callus. Differences in 17, 18.4 and 25 kDa protein concentrations were also observed in browning and non-browning callus using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/8009 |
| Collection | 热带植物资源可持续利用重点实验室特色文献_小桐子相关文献 |
| Recommended Citation GB/T 7714 | He, Y.. Changes in morphology and biochemical indices in browning callus derived from Jatropha curcas hypocotyls[J]. Plant Cell Tissue and Organ Culture,2009,98(1):42325. |
| APA | He, Y..(2009).Changes in morphology and biochemical indices in browning callus derived from Jatropha curcas hypocotyls.Plant Cell Tissue and Organ Culture,98(1),42325. |
| MLA | He, Y.."Changes in morphology and biochemical indices in browning callus derived from Jatropha curcas hypocotyls".Plant Cell Tissue and Organ Culture 98.1(2009):42325. |
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| Changes in morpholog（338KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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