Analysis of Genetic Diversity in Pongamia Pongamia pinnata (L) Pierrre using AFLP Markers
Thudi, M.
2010
Source PublicationJournal of Plant Biochemistry and Biotechnology
ISSN0971-7811
Volume19Issue:2Pages:209-216
AbstractIn recent years, Pongamia has been considered as important renewable source of biodiesel, however not much molecular information is available in this species. Molecular characterization of this legume tree will enhance our understanding in improving the optimal yields of oil through breeding and enable us to meet the future demands for biodiesel. To assess the molecular genetic diversity in 48 Pongamia pinnata accessions collected from six different states of India, amplified fragment length polymorphism (AFLP) marker system was employed. Five AFLP primer combinations produced 520 discernible fragments, of which 502 (96.5%) were polymorphic. AFLP primer informativeness was estimated evaluating four parameters namely polymorphism information content (PIC), effective multiplex ratio (EMR), marker index (MI) and resolving power (RP). In total, 51 unique fragments were detected of which 19 unique fragments were observed with primer combination E-ACG / M-CTA. Although neighbour joining (NJ) method did not group accessions strictly according to their region of collection, a good level of genetic diversity was observed in examined germplasm. However, accessions collected from Karnataka showed comparatively higher diversity than accessions from other states. The diverse accessions identified in this study may be useful in Pongamia pinnata improvement to meet the future demands of biodiesel.
Document Type期刊论文
Identifierhttps://ir.xtbg.ac.cn/handle/353005/7822
Collection热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
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GB/T 7714
Thudi, M.. Analysis of Genetic Diversity in Pongamia Pongamia pinnata (L) Pierrre using AFLP Markers[J]. Journal of Plant Biochemistry and Biotechnology,2010,19(2):209-216.
APA Thudi, M..(2010).Analysis of Genetic Diversity in Pongamia Pongamia pinnata (L) Pierrre using AFLP Markers.Journal of Plant Biochemistry and Biotechnology,19(2),209-216.
MLA Thudi, M.."Analysis of Genetic Diversity in Pongamia Pongamia pinnata (L) Pierrre using AFLP Markers".Journal of Plant Biochemistry and Biotechnology 19.2(2010):209-216.
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