Institutional Repository of Xishuangbanna Tropical Botanical Garden
| Efficient In vitro Plant Regeneration Protocol from Leaf Explant of Jatropha curcas L - A Promising Biofuel Plant | |
| Kumar, S. | |
| 2010 | |
| Source Publication | Journal of Plant Biochemistry and Biotechnology
 |
| ISSN | 0971-7811 |
| Volume | 19Issue:2Pages:275-277 |
| Abstract | An efficient in vitro plant regeneration from leaf-disc culture of Jatropha curcas L has been established. Adventitious shoot buds along with callus were induced from leaves of 2-year-old J. curcas plants cultured on Murashige and Skoog's (MS) medium supplemented with TDZ (2 mu M) BAP (2 mu M) and IBA (1 mu M), wherein 83.3% leaf explants responded. The multiplication of shoots was achieved from the adventitious shoot buds after transferring them to shoot induction medium. The highest number of shoots (9.7/explant) was achieved after 8 weeks of culture on MS medium containing 3 mu M of BAP. The well-developed shoots were rooted on MS medium supplemented with IBA (1.5 mu M) with the rooting frequency of 53.3%. Addition of phloroglucinol (200 mu M) to the medium enhanced the frequency of rooting to 76.7%. Regenerated plantlets were successfully transferred to field after initial acclimatization. |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/7713 |
| Collection | 热带植物资源可持续利用重点实验室特色文献_小桐子相关文献 |
| Recommended Citation GB/T 7714 | Kumar, S.. Efficient In vitro Plant Regeneration Protocol from Leaf Explant of Jatropha curcas L - A Promising Biofuel Plant[J]. Journal of Plant Biochemistry and Biotechnology,2010,19(2):275-277. |
| APA | Kumar, S..(2010).Efficient In vitro Plant Regeneration Protocol from Leaf Explant of Jatropha curcas L - A Promising Biofuel Plant.Journal of Plant Biochemistry and Biotechnology,19(2),275-277. |
| MLA | Kumar, S.."Efficient In vitro Plant Regeneration Protocol from Leaf Explant of Jatropha curcas L - A Promising Biofuel Plant".Journal of Plant Biochemistry and Biotechnology 19.2(2010):275-277. |
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| Efficient In vitro P（69KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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