Institutional Repository of Xishuangbanna Tropical Botanical Garden
| Establishment of an Agrobacteriuim-mediated cotyledon disc transformation method for Jatropha curcas | |
| Li, M. R. | |
| 2008 | |
| Source Publication | Plant Cell Tissue and Organ Culture
 |
| ISSN | 0167-6857 |
| Volume | 92Issue:2Pages:173-181 |
| Abstract | Jatropha curcas contains high amounts of oil in its seed and has been considered for bio-diesel production. A transformation procedure for J. curcas has been established for the first time via Agrobacterium tumefaciens infection of cotyledon disc explants. The results indicated that the efficiency of transformation using the strain LBA4404 and phosphinothricin for selection was an improvement over that with the strain EHA105 and hygromycin. About 55% of the cotyledon explants produced phosphinothricin-resistant calluses on Murashige and Skoog (MS) medium supplemented with 1.5 mg 1(-1) benzyladenine (BA), 0.05 mg 1(-1) 3-indolebutyric acid (IBA), 1 mg 1(-1) phosphinothricin and 500 mg 1(-1) cefotaxime after 4 weeks. Shoots were regenerated following transfer of the resistant calli to shoot induction medium containing 1.5 mg 1(-1) BA, 0.05 mg 1(-1) IBA, 0.5 mg 1(-1) gibberellic acid (GA3), 1 mg 1(-1) phosphinothricin and 250 mg 1(-1) cefotaxime, and about 33% of the resistant calli differentiated into shoots. Finally, the resistant shoots were rooted on 1/2 MS media supplemented with 0.3 mg 1(-1) IBA at a rate of 78%. The transgenic nature of the transformants was demonstrated by the detection of beta-glucuronidase activity in the primary transformants and by PCR and Southern hybridization analysis. 13% of the total inoculated explants produced transgenic plants after approximately 4 months. The procedure described will be useful for both, the introduction of desired genes into J. curcas and the molecular analysis of gene function. |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/7530 |
| Collection | 热带植物资源可持续利用重点实验室特色文献_小桐子相关文献 |
| Recommended Citation GB/T 7714 | Li, M. R.. Establishment of an Agrobacteriuim-mediated cotyledon disc transformation method for Jatropha curcas[J]. Plant Cell Tissue and Organ Culture,2008,92(2):173-181. |
| APA | Li, M. R..(2008).Establishment of an Agrobacteriuim-mediated cotyledon disc transformation method for Jatropha curcas.Plant Cell Tissue and Organ Culture,92(2),173-181. |
| MLA | Li, M. R.."Establishment of an Agrobacteriuim-mediated cotyledon disc transformation method for Jatropha curcas".Plant Cell Tissue and Organ Culture 92.2(2008):173-181. |
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