Institutional Repository of Xishuangbanna Tropical Botanical Garden
| Engineering geminivirus resistance in Jatropha curcus | |
| Ye, J. | |
| 2014 | |
| Source Publication | Biotechnology for Biofuels
 |
| ISSN | 1754-6834 |
| Volume | 7 |
| Abstract | Background: Jatropha curcus is a good candidate plant for biodiesel production in tropical and subtropical regions. However, J. curcus is susceptible to the geminivirus Indian cassava mosaic virus (ICMV), and frequent viral disease outbreaks severely limit productivity. Therefore the development of J. curcus to carry on durable virus resistance remains crucial and poses a major biotechnological challenge. Results: We generated transgenic J. curcus plants expressing a hairpin, double-stranded (ds) RNA with sequences homologous to five key genes of ICMV-Dha strain DNA-A, which silences sequence-related viral genes thereby conferring ICMV resistance. Two rounds of virus inoculation were conducted via vacuum infiltration of ICMV-Dha. The durability and heritability of resistance conferred by the dsRNA was further tested to ascertain that T1 progeny transgenic plants were resistant to the ICMV-SG strain, which shared 94.5% nucleotides identity with the ICMV-Dha strain. Quantitative PCR analysis showed that resistant transgenic lines had no detectable virus. Conclusions: In this study we developed transgenic J. curcus plants to include a resistance to prevailing geminiviruses in Asia. These virus-resistant transgenic J. curcus plants can be used in various Jatropha breeding programs. |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/7310 |
| Collection | 热带植物资源可持续利用重点实验室特色文献_小桐子相关文献 |
| Recommended Citation GB/T 7714 | Ye, J.. Engineering geminivirus resistance in Jatropha curcus[J]. Biotechnology for Biofuels,2014,7. |
| APA | Ye, J..(2014).Engineering geminivirus resistance in Jatropha curcus.Biotechnology for Biofuels,7. |
| MLA | Ye, J.."Engineering geminivirus resistance in Jatropha curcus".Biotechnology for Biofuels 7(2014). |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Engineering geminivi（2040KB） | 开放获取 | CC BY-NC-SA | View Download | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment