Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis delta-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus)

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	Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis delta-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus)
	Gu, K. Y.
	2014
Source Publication	Biotechnology for Biofuels
ISSN	1754-6834
Volume	7 Issue:x
Abstract	Background: The potential biofuel plant Jatropha curcas L. is affected by larvae of Archips micaceanus (Walker), a moth of the family Tortricidae. The hybrid Bacillus thuringiensis (Bt) delta-endotoxin protein Cry1Ab/1Ac confers resistance to lepidopteran insects in transgenic rice. Results: Here, we report the production of a marker-free transgenic line of J. curcas (L10) expressing Cry1Ab/1Ac using Agrobacterium-mediated transformation and a chemically regulated, Cre/loxP-mediated DNA recombination system. L10 carries a single copy of marker-free T-DNA that contains the Cry1Ab/1Ac gene under the control of a maize phosphoenolpyruvate carboxylase gene promoter (P-Pepc:Cry1Ab/1Ac:T-Nos). The P-Pepc:Cry1Ab/1Ac:T-Nos gene was highly expressed in leaves of L10 plants. Insecticidal bioassays using leaf explants of L10 resulted in 80-100% mortality of larvae of A. micaceanus at 4 days after infestation. Conclusion: The results demonstrate that the hybrid Bt delta-endotoxin protein Cry1Ab/1Ac expressed in Jatropha curcas displays strong insecticidal activity to A. micaceanus. The marker-free transgenic J. curcas line L10 can be used for breeding of insect resistance to A. micaceanus.
Document Type	期刊论文
Identifier	https://ir.xtbg.ac.cn/handle/353005/7231
Collection	热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
Recommended Citation GB/T 7714	Gu, K. Y.. Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis delta-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus)[J]. Biotechnology for Biofuels,2014,7(x).
APA	Gu, K. Y..(2014).Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis delta-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus).Biotechnology for Biofuels,7(x).
MLA	Gu, K. Y.."Production of marker-free transgenic Jatropha curcas expressing hybrid Bacillus thuringiensis delta-endotoxin Cry1Ab/1Ac for resistance to larvae of tortrix moth (Archips micaceanus)".Biotechnology for Biofuels 7.x(2014).

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