| Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis | |
| Xu, RH; Yang, TQ; Wang, RL; Liu, AZ | |
| 2014 | |
| Source Publication | FUNCTIONAL PLANT BIOLOGY
 |
| ISSN | 1445-4408 |
| Volume | 41Issue:3Pages:321-329 |
| Abstract | Diacylglycerol acyltransferases (DGATs) catalyse the final step of triacylglycerol (TAG) biosynthesis of the Kennedy pathway, and play a critical role during TAG accumulation in developing oleaginous seeds. In this study, the molecular cloning and characterisation of two DGAT genes, JcDGAT1 and JcDGAT2, from jatropha (Jatropha curcas L., a potential biodiesel plant) is presented. Using heterogonous overexpression techniques, both JcDGAT1 and JcDGAT2 were able to restore TAG biosynthesis in a yeast mutant H1246 strain, and enhance the quantity of TAG biosynthesis by 16.6 and 14.3%, respectively, in strain INVSc1. In transgenic tobacco, overexpression of JcDGAT1 and JcDGAT2 resulted in an increase in seed oil content of, respectively, 32.8 and 31.8%. Further, the functional divergence of JcDGAT1 and JcDGAT2 in TAG biosynthesis was demonstrated by comparing the fatty acid compositions in both the transgenic yeast and tobacco systems. In particular, JcDGAT2 incorporated a 2.5-fold higher linoleic acid content into TAG than JcDGAT1 in transgenic yeast and exhibited a significant linoleic acid substrate preference in both yeast and tobacco. This study provides new insights in understanding the molecular mechanisms of DGAT genes underlying the biosynthesis of linoleic acids and TAG in plants. |
| Keyword | Diacylglycerol Acyltransferase Fatty Acid Composition Oil Content Triacylglycerols |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/5091 |
| Collection | 分子遗传和育种研究组 |
| Recommended Citation GB/T 7714 | Xu, RH,Yang, TQ,Wang, RL,et al. Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis[J]. FUNCTIONAL PLANT BIOLOGY,2014,41(3):321-329. |
| APA | Xu, RH,Yang, TQ,Wang, RL,&Liu, AZ.(2014).Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis.FUNCTIONAL PLANT BIOLOGY,41(3),321-329. |
| MLA | Xu, RH,et al."Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis".FUNCTIONAL PLANT BIOLOGY 41.3(2014):321-329. |
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