| The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum | |
Luo, M; Ding, LW; Ge, ZJ; Wang, ZY; Hu, BL; Yang, XB; Sun, QY; Xu, ZF*
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| 2012 | |
| Source Publication | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| Volume | 13Issue:11Pages:15162-15176 |
| Abstract | Proteinase inhibitors play an important role in plant resistance of insects and pathogens. In this study, we characterized the serine proteinase inhibitor SaPIN2b, which is constitutively expressed in Solanum americanum trichomes and contains two conserved motifs of the proteinase inhibitor II (PIN2) family. The recombinant SaPIN2b (rSaPIN2b), which was expressed in Escherichia coli, was demonstrated to be a potent proteinase inhibitor against a panel of serine proteinases, including subtilisin A, chymotrypsin and trypsin. Moreover, rSaPIN2b also effectively inhibited the proteinase activities of midgut trypsin-like proteinases that were extracted from the devastating pest Helicoverpa armigera. Furthermore, the overexpression of SaPIN2b in transgenic tobacco plants resulted in enhanced resistance against H. armigera. Taken together, our results demonstrated that SaPIN2b is a potent serine proteinase inhibitor that may act as a protective protein in plant defense against insect attacks. |
| Keyword | Pin2 Proteinase Inhibitor Solanum Americanum Insect Resistance Trichome |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/3869 |
| Collection | 能源植物分子育种研究组 |
| Recommended Citation GB/T 7714 | Luo, M,Ding, LW,Ge, ZJ,et al. The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(11):15162-15176. |
| APA | Luo, M.,Ding, LW.,Ge, ZJ.,Wang, ZY.,Hu, BL.,...&Xu, ZF*.(2012).The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,13(11),15162-15176. |
| MLA | Luo, M,et al."The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.11(2012):15162-15176. |
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