| Integrated transcriptomic, transcriptional factors, and protein interaction reveal the regulatory mechanisms of flowering time in rice (Oryza sativa L.) | |
| Sohail, Amir1; Shah, Liaqat2; Shah, Syed Mehar Ali1; Abbas, Adil3; Ali, Shahzad4; Manzoor5 | |
| 2025 | |
| Source Publication | TRANSGENIC RESEARCH
 |
| ISSN | 0962-8819 |
| Volume | 34Issue:1Pages:- |
| Abstract | Appropriate flowering time is important for rice regional adaptation and optimum rice production, but little is known about the omics of heading date in rice. Here, we studied omics including transcriptome, proteome and transcriptional factors to identify regulatory genes related to flowering time. A total of 1402 differentially expressed genes (DEGs, 721 up-regulated and 681 down-regulated) were detected in wild and mutant. These transcripts are classified according to biological processes, cellular components, and molecular functions. Among these differentially expressed genes, many transcription factor genes demonstrated multiple regulatory pathways involved in flowering time. Gene expression analysis showed that Os03g0122600 (OsMADS50), Os08g0105000 (Ehd3), Os06g0275000 (Hd1) were expressed higher and Os06g0199500 (OsHAL3), Os06g0498800 (OsMFT1), Os08g0105000 (Ehd3), Os06g0157700 (Hd3a), and Os02g0731700 (Ghd2), were expressed lower in wild compared to mutant, which are the key genes that regulate the flowering in rice. In addition, Ghd7 interacted with Os10g30860 and Os12g08260 using yeast two-hybrid assay. We identified 28 potential Ghd7 transcriptional regulators using the transcription factor-centered yeast one hybrid (TF-Centered Y1H) assay. Taken together, this study developed a new set of genomic resources to identify and characterize genes, proteins, and motifs associated with flowering time. |
| Keyword | Rice Flowering time RNA-seq TF-Centered Y1H Yeast two-hybrid (Y2H) |
| Subject Area | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| DOI | 10.1007/s11248-025-00439-8 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001469337900001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/15711 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Chinese Acad Sci, Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Kunming 650223, Peoples R China 2.Univ Agr, Dept Plant Breeding & Genet, Peshawar, Pakistan 3.Mir Chakar Khan Rind Univ, Dept Agr, Sibi 82000, Pakistan 4.Univ Sialkot, Fac Sci, Dept Biotechnol, Sialkot, Punjab, Pakistan 5.Hazara Univ, Dept Agr, Mansehra 21120, Pakistan 6.Chinese Acad Sci, Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Xishuangbanna 666303, Peoples R China |
| Recommended Citation GB/T 7714 | Sohail, Amir,Shah, Liaqat,Shah, Syed Mehar Ali,et al. Integrated transcriptomic, transcriptional factors, and protein interaction reveal the regulatory mechanisms of flowering time in rice (Oryza sativa L.)[J]. TRANSGENIC RESEARCH,2025,34(1):-. |
| APA | Sohail, Amir,Shah, Liaqat,Shah, Syed Mehar Ali,Abbas, Adil,Ali, Shahzad,&Manzoor.(2025).Integrated transcriptomic, transcriptional factors, and protein interaction reveal the regulatory mechanisms of flowering time in rice (Oryza sativa L.).TRANSGENIC RESEARCH,34(1),-. |
| MLA | Sohail, Amir,et al."Integrated transcriptomic, transcriptional factors, and protein interaction reveal the regulatory mechanisms of flowering time in rice (Oryza sativa L.)".TRANSGENIC RESEARCH 34.1(2025):-. |
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| Integrated transcrip（1448KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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