| Evening complex proteins antagonize ABI3 and ABI5 to temporally regulate abscisic acid signaling and seed germination | |
Tang, Ruyu1; Yang, Jiajia1; Han, Xiao; He, Kunrong2; Zhang, Cuiping; Yang, Milian; Zhang, Juping1; Huang, Zhichong; Ye, Jingwen; Xu, Tingting1; Yu, Chunlan; Du, Jiancan; Fu, Qiantang; Hu, Yanru3,4
| |
| 2025 | |
| Source Publication | PLANT CELL
 |
| ISSN | 1040-4651 |
| Volume | 37Issue:8Pages:- |
| Abstract | Seed germination and postgerminative growth are precisely regulated by multiple signals. In Arabidopsis thaliana, the phytohormone abscisic acid (ABA) suppresses these processes and several circadian clock-associated proteins mediate ABA responses. Nevertheless, whether seed germination is controlled by temporal signals under diurnal conditions remains obscure, as do the associated underlying molecular mechanisms. Here, we found that the germination of wild-type seeds varies with time of release from cold stratification (i.e. transferred to 22 degrees C) under diurnal conditions upon ABA, salinity, or osmotic stress exposure. Additionally, the evening complex (EC) components EARLY FLOWERING 3 (ELF3), ELF4, and LUX ARRHYTHMO (LUX) attenuate ABA signaling. Notably, time-dependent seed germination relies on these EC components and other core clock proteins. ELF3, ELF4, and LUX physically interact with and act genetically upstream of ABSCISIC ACID INSENSITIVE3 (ABI3) and ABI5, two crucial transcriptional activators of ABA signaling. ELF3, ELF4, and LUX repress the function and accumulation of ABI3 and ABI5. Consistent with these results, ABI3 and ABI5 are essential for the time-based modulation of seed germination. Our findings highlight the critical effects of temporal signals on seed germination and clarify the mechanism through which the EC components antagonize ABI3 and ABI5 to facilitate the crosstalk between the clock and ABA signaling pathways. Evening complex proteins bind to and suppress the transcription factors ABI3 and ABI5 to integrate the circadian clock and abscisic acid signaling pathways during the temporal regulation of seed germination. |
| Keyword | PSEUDO-RESPONSE REGULATORS ARABIDOPSIS CIRCADIAN CLOCK TRANSCRIPTION FACTOR GENE-EXPRESSION FLOWERING TIME NEGATIVE REGULATOR STRESS-RESPONSE ROOT-GROWTH E3 LIGASES ABA |
| Subject Area | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| DOI | 10.1093/plcell/koaf189 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001559890800001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/15688 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Chinese Acad Sci, CAS Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Kunming 650223, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 3.Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, State Key Lab Tree Genet & Breeding, Nanjing 210037, Peoples R China 4.Hunan Univ, Coll Biol,Hunan Key Lab Plant Funct Genom & Dev Re, Hunan Res Ctr Basic Discipline Cell Signaling, State Key Lab Chemo & Biosensing, Changsha 410082, Peoples R China 5.Yuelushan Lab, Changsha 410128, Peoples R China |
| Recommended Citation GB/T 7714 | Tang, Ruyu,Yang, Jiajia,Han, Xiao,et al. Evening complex proteins antagonize ABI3 and ABI5 to temporally regulate abscisic acid signaling and seed germination[J]. PLANT CELL,2025,37(8):-. |
| APA | Tang, Ruyu.,Yang, Jiajia.,Han, Xiao.,He, Kunrong.,Zhang, Cuiping.,...&Hu, Yanru.(2025).Evening complex proteins antagonize ABI3 and ABI5 to temporally regulate abscisic acid signaling and seed germination.PLANT CELL,37(8),-. |
| MLA | Tang, Ruyu,et al."Evening complex proteins antagonize ABI3 and ABI5 to temporally regulate abscisic acid signaling and seed germination".PLANT CELL 37.8(2025):-. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Evening complex prot（3632KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment