| Physiological, transcriptomic and metabolomic analyses reveal the mechanism of CuO and silicon nanoparticles involved in Polygonatum kingianum response to root rot | |
Xu, Xi1; Wan, Jinpeng1 ; Liu, Guizhou1; Lu, Chengkai1; Mao, Xinyu1; Wu, Jinglin1; Liu, Hanying1; Ding, Yong; Xu, Peng1
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| 2025 | |
| Source Publication | CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
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| ISSN | 2196-5641 |
| Volume | 12Issue:1Pages:- |
| Abstract | BackgroundCopper oxide nanoparticles (CuNPs) and silicon nanoparticles (SiNPs) play a crucial role in enhancing plant growth and development under stress conditions, making them valuable tools in sustainable agriculture. However, the mechanisms by which CuNPs and SiNPs influence plant responses to root rot remain poorly understood. This study integrated physiological, transcriptomic, and metabolomic analyses to elucidate the potential mechanisms of Polygonatum kingianum, a well-known medicinal plant, in response to root rot induced by Fusarium oxysporum.ResultsThe results demonstrated that F. oxysporum inoculation severely induced root rot in P. kingianum, leading to rhizome decay, reduced root biomass, and impaired leaf photosynthetic capacity. In contrast, foliar application of CuNPs and SiNPs significantly enhanced P. kingianum against rhizome rot, with relative therapeutic effects increasing by 48.68% and 50.31%, respectively, thereby showing an increment in the growth of the seedling. In addition, these nanoparticles modulated the balance of ROS and antioxidant abundance, improved mineral element content, and thereby enhanced photosynthetic ability under root rot conditions. CuNPs and SiNPs reprogrammed differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) involved in photosynthesis, carbon fixation via the Calvin cycle, glycolysis/gluconeogenesis, starch and sucrose metabolism, the TCA cycle, glutathione metabolism, flavonoid metabolism, and phenylpropanoid metabolism, thus modulating P. kingianum against rhizome rot through primary and secondary metabolic pathways. Combined KEGG enrichment analysis of DEGs and DAMs revealed that cysteine and methionine metabolism, ABC transporters, flavonoid biosynthesis, purine metabolism, and plant hormone signal transduction were enriched upon CuNPs treatment, whereas cysteine and methionine metabolism, pyruvate metabolism, and galactose metabolism were significantly enriched upon SiNPs treatment. A Pearson coefficient analysis showed that 22 genes were positively correlated with the disease index under CuNP treatment, while 27 genes were positively correlated under SiNP treatment. Furthermore, 27 common DAMs related to flavonoid metabolism, isoflavonoid metabolism, and amino acid metabolism were identified in seedlings treated with both CuNPs and SiNPs.ConclusionsCuNPs or SiNPs enhanced the resistance of P. kingianum to root rot through the regulation of osmoprotectant and ROS homeostasis, modulation of mineral element accumulation, and reprogramming of key transcriptional and metabolic pathways, highlighting the potential of NPs in preventing root diseases in medicinal plants. |
| Keyword | Copper oxide nanoparticles Silicon nanoparticles Polygonatum kingianum Root rot Medicinal plant |
| Subject Area | Agriculture, Multidisciplinary |
| DOI | 10.1186/s40538-025-00821-y |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001537098000001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/15604 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Southwest Forestry Univ, Forest Resources Exploitat & Utilizat Engn Res Ctr, Coll Biol Sci & Food Engn, Kunming 650224, Yunnan, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Menglun 666303, Yunnan, Peoples R China |
| Recommended Citation GB/T 7714 | Xu, Xi,Wan, Jinpeng,Liu, Guizhou,et al. Physiological, transcriptomic and metabolomic analyses reveal the mechanism of CuO and silicon nanoparticles involved in Polygonatum kingianum response to root rot[J]. CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE,2025,12(1):-. |
| APA | Xu, Xi.,Wan, Jinpeng.,Liu, Guizhou.,Lu, Chengkai.,Mao, Xinyu.,...&Xu, Peng.(2025).Physiological, transcriptomic and metabolomic analyses reveal the mechanism of CuO and silicon nanoparticles involved in Polygonatum kingianum response to root rot.CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE,12(1),-. |
| MLA | Xu, Xi,et al."Physiological, transcriptomic and metabolomic analyses reveal the mechanism of CuO and silicon nanoparticles involved in Polygonatum kingianum response to root rot".CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE 12.1(2025):-. |
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| Physiological, trans（12477KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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