| Unveiling the catalytical roles of three glycosyltransferases in triterpenoid saponins in quinoa | |
Zhang, Yiming1,2; Zhu, Peng1; Wu, Long3; Li, Zhaohui1; Huang, Yongshun1; Wang, Mingyu1; Gu, Kaiying1; Wang, Peng4; Li, Jun2; Wan, Huihua2; Zhang, Hailong5; Chen, Jianghua5 ; He, Ruikun6; Sun, Wei2; Li, Lixin1
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| 2025 | |
| Source Publication | PLANT BIOTECHNOLOGY JOURNAL
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| ISSN | 1467-7644 |
| Volume | xIssue:xPages:- |
| Abstract | Saponins constitute a class of characteristic compounds within quinoa grains. In this study, metabolomic analysis identified 20 triterpene saponins, the majority of which were exclusively localized within the pericarp. Furthermore, three crucial glycosyltransferases were identified in quinoa. CqCsl2 was characterized as a 3-O-glucuronosyltransferase, CqUGT73FG1 as a 2 '-O-glucosyltransferase and CqUGT74BB3 as a C-28 carboxyl glucosyltransferase involved in triterpenoid glycosylation. To elucidate the catalytic mechanisms of CqUGT74BB3, molecular docking simulations and site-directed mutagenesis experiments revealed His13 and Asp104 as critical catalytic residues. Moreover, C-28 carboxyl glycosylation of oleanolic acid was observed to occur only when the C-3 position was modified with either mono-glucuronidation or di-O-glycosylation. This specific modification strategically positions the C-28 carboxyl group in close proximity to the catalytic centre of CqUGT74BB3 (His13-Asp104), enabling the glycosylation reaction. We successfully synthesized key triterpene saponins, exemplified by ginsenoside Ro, in tobacco. These findings have significant implications for future metabolic engineering endeavours aimed at developing quinoa varieties with enhanced nutritional and medicinal attributes. |
| Keyword | UDP-GLYCOSYLTRANSFERASES ACID IDENTIFICATION RESISTANCE DIVERSITY GENOME PLANTS |
| Subject Area | Biotechnology & Applied Microbiology ; Plant Sciences |
| DOI | 10.1111/pbi.70214 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001517347900001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/14780 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Minist Educ, Key Lab Saline alkali Vegetat Ecol Restorat, Harbin, Peoples R China 2.Northeast Forestry Univ, Coll Life Sci, Harbin, Peoples R China 3.China Acad Chinese Med Sci, Inst Chinese Mat Med, State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing, Peoples R China 4.Create Beijing Technol Co Ltd, Beijing, Peoples R China 5.Northwest A&F Univ, Coll Life Sci, Yangling, Peoples R China 6.Chinese Acad Sci, Key Lab Top Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Kunming, Yunnan, Peoples R China 7.By Hlth Inst Nutr & Hlth, Guangzhou, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Yiming,Zhu, Peng,Wu, Long,et al. Unveiling the catalytical roles of three glycosyltransferases in triterpenoid saponins in quinoa[J]. PLANT BIOTECHNOLOGY JOURNAL,2025,x(x):-. |
| APA | Zhang, Yiming.,Zhu, Peng.,Wu, Long.,Li, Zhaohui.,Huang, Yongshun.,...&Li, Lixin.(2025).Unveiling the catalytical roles of three glycosyltransferases in triterpenoid saponins in quinoa.PLANT BIOTECHNOLOGY JOURNAL,x(x),-. |
| MLA | Zhang, Yiming,et al."Unveiling the catalytical roles of three glycosyltransferases in triterpenoid saponins in quinoa".PLANT BIOTECHNOLOGY JOURNAL x.x(2025):-. |
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| Unveiling the cataly（2201KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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