| Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress | |
Zhao, Jianping5; Yao, Baolin4; Peng, Ziai; Yang, Xinyue; Li, Kuixiu; Zhang, Xiaoyan; Zhu, Haiyan; Zhou, Xuan; Wang, Meixian; Jiang, Lihui; He, Xie; Liang, Yan6; Zhan, Xiaoping7; Wang, Xiaoran; Dai, Yuliang; Yang, Yanfen; Yang, Ao; Dong, Man; Shi, Suni; Lu, Man; Zhao, Yi; Shen, Mingyun; Guo, Liwei2,3; Liu, Changning4 ; Zhang, Hongji1,8; Yu, Decai2,3; Du, Yunlong2,3
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| 2025 | |
| Source Publication | HORTICULTURAL PLANT JOURNAL
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| ISSN | 2095-9885 |
| Volume | 11Issue:2Pages:706-720 |
| Abstract | The formation of root system architecture (RSA) plays a crucial role in plant growth. OsDRO1 is known to have a function in controlling RSA in rice, however, the role of potato StDRO2, a homolog of rice OsDRO1, in root growth remains unclear. In this study, we obtained potato dro2 mutant lines by Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-Associated 9 (CRISPR/Cas9)-mediated genome editing system. The mutant lines were generated from a splicing defect of the StDRO2 intron 1, which causes a nonsense mutation in StDRO2. Furthermore, the secondary structure of StDRO2 mRNA analyzed with RNAfold WebServer was altered in the dro2 mutant. Mutation of StDRO2 conveys potato adaptation through changing the RSA via alteration of auxin transport under drought stress. The potato dro2 lines showed higher plant height, longer root length, smaller root growth angle and increased tuber weight than the wild-type. The alteration of RSA was associated with a disturbance of IAA distribution in the dro2 mutant, and the levels of StPIN7 and StPIN10 detected by using real-time PCR were up-regulated in the roots of potato dro2 lines grown under drought stress. Moreover, the microRNAs (miRNAs) PmiREN024536 and PmiREN024486 targeted the StDRO2 gene, and auxin positively and negatively regulated the expression of StDRO2 and the miRNAs PmiREN024536 and PmiREN024486, respectively, in the potato roots. Our data shows that a regulatory network involving auxin, StDRO2, PmiREN024536 and PmiREN024486 can control RSA to convey potato fitness under drought stress. |
| Keyword | Potato Root system architecture StDRO2 Drought stress Auxin Splicing MicroRNA |
| Subject Area | Plant Sciences ; Agriculture |
| DOI | 10.1016/j.hpj.2023.11.003 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001439179200001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/14724 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Yunnan Agr Univ, Coll Plant Protect, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Agr Univ, Coll Landscape & Hort, Kunming 650201, Yunnan, Peoples R China 3.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Yu, Kunming 650201, Yunnan, Peoples R China 4.Yunnan Agr Univ, Coll Plant Protect, Key Lab Agrobiodivers & Pest Management Educ Minis, Kunming 650201, Yunnan, Peoples R China 5.Chinese Acad Sci, CAS Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Menglun 666300, Yunnan, Peoples R China 6.Xuanhan Cty Plant Quarantine Stn, Dazhou 635000, Sichuan, Peoples R China 7.Yuguopu Dist Agr Comprehens Serv Ctr, Mengzi 661100, Yunnan, Peoples R China 8.Ninger Cty Plant Protect & Plant Quarantine Stn, Puer 665000, Yunnan, Peoples R China 9.Yunnan Agr Univ, Coll Landscape & Hort, Key Lab Vegetable Biol Yunnan Prov, Kunming 650201, Yunnan, Peoples R China |
| Recommended Citation GB/T 7714 | Zhao, Jianping,Yao, Baolin,Peng, Ziai,et al. Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress[J]. HORTICULTURAL PLANT JOURNAL,2025,11(2):706-720. |
| APA | Zhao, Jianping.,Yao, Baolin.,Peng, Ziai.,Yang, Xinyue.,Li, Kuixiu.,...&Du, Yunlong.(2025).Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress.HORTICULTURAL PLANT JOURNAL,11(2),706-720. |
| MLA | Zhao, Jianping,et al."Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress".HORTICULTURAL PLANT JOURNAL 11.2(2025):706-720. |
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| Splicing defect of S（6227KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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