| The chromosome-scale genomes of two Tinospora species reveal differential regulation of the MEP pathway in terpenoid biosynthesis | |
Chen, Zhiyu4; Xun, Lan6; Lu, Yunyan6; Yang, Xingyu3,4; Chen, Minghui2; Yang, Tianyu4; Mei, Zhinan7; Yang, Yunqiang2,3,4; Yang, Xuefei1,4,5; Yang, Yongping2,3,4
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| 2025 | |
| Source Publication | BMC BIOLOGY
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| ISSN | 1741-7007 |
| Volume | 23Issue:1Pages:- |
| Abstract | BackgroundThe relationship between gene family expansion and the resulting changes in plant phenotypes has shown remarkable complexity during the evolution. The gene family expansion has contributed to the diversity in plant phenotypes, specifically metabolites through neo-functionalization and sub-functionalization. However, the negative regulatory effects associated with the gene family expansion remain poorly understood.ResultsHere, we present the chromosome-scale genomes of Tinospora crispa and Tinospora sinensis. Comparative genomic analyses demonstrated conserved chromosomal evolution within the Menispermaceae family. KEGG analysis revealed a significant enrichment of genes related to terpenoid biosynthesis in T. sinensis. However, T. crispa exhibited a higher abundance of terpenoids compared to T. sinensis. Detailed analysis revealed the expansion of genes encoding 1-hydroxy-2-methyl 2-(E)-butenyl 4-diphosphate synthase (HDS), a key enzyme in the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway of terpenoid biosynthesis in T. sinensis. TsiHDS4 retained the ancestral function of converting methylerythritol cyclic diphosphate (MEcPP) to (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP). However, the noncanonical CDS-derived small peptide TsiHDS5 was shown to interact with TsiHDS4, inhibiting its catalytic activity. This interaction reduced the levels of HMBPP and isopentenyl pyrophosphate (IPP), which represent key substrates for downstream terpenoid biosynthesis.ConclusionsThese findings offer clues to decipher the variations in the MEP pathway of terpenoid biosynthesis between T. crispa and T. sinensis and form a basis for further detailed research on the negative regulation of expanded genes. |
| Keyword | Tinospora Comparative genomics Terpenoid biosynthesis MEP pathway 1-hydroxy-2-methyl 2-(E)-butenyl 4-diphosphate synthase Gene family expansion |
| Subject Area | Life Sciences & Biomedicine - Other Topics |
| DOI | 10.1186/s12915-025-02185-z |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001449039000002 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/14692 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Chinese Acad Sci, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Key Lab Econ Plants & Biotechnol, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 3.Chinese Acad Sci, Yunnan Int Joint Lab Conservat & Utilizat Trop Tim, Xishuangbanna Trop Bot Garden, Mengla 666303, Yunnan, Peoples R China 4.Chinese Acad Sci, CAS Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Mengla 666303, Yunnan, Peoples R China 5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 6.Chinese Acad Sci, Southeast Asia Biodivers Res Inst, Nay Pyi Taw 05282, Myanmar 7.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China 8.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China |
| Recommended Citation GB/T 7714 | Chen, Zhiyu,Xun, Lan,Lu, Yunyan,et al. The chromosome-scale genomes of two Tinospora species reveal differential regulation of the MEP pathway in terpenoid biosynthesis[J]. BMC BIOLOGY,2025,23(1):-. |
| APA | Chen, Zhiyu.,Xun, Lan.,Lu, Yunyan.,Yang, Xingyu.,Chen, Minghui.,...&Yang, Yongping.(2025).The chromosome-scale genomes of two Tinospora species reveal differential regulation of the MEP pathway in terpenoid biosynthesis.BMC BIOLOGY,23(1),-. |
| MLA | Chen, Zhiyu,et al."The chromosome-scale genomes of two Tinospora species reveal differential regulation of the MEP pathway in terpenoid biosynthesis".BMC BIOLOGY 23.1(2025):-. |
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