| Genome-Wide Characterization of the INDETERMINATE DOMAIN (IDD) Zinc Finger Gene Family in Solanum lycopersicum and the Functional Analysis of SlIDD15 in Shoot Gravitropism | |
Wu, Huan1; Liu, Mingli1; Fang, Yuqi1; Yang, Jing1; Xie, Xiaoting1,2; Zhang, Hailong1; Zhou, Dian1; Zhou, Yueqiong1,2; He, Yexin1; Chen, Jianghua1,2 ; Bai, Quanzi1,2
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| 2024 | |
| Source Publication | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| ISSN | 1661-6596 |
| Volume | 25Issue:19Pages:- |
| Abstract | The plant-specific IDD transcription factors (TFs) are vital for regulating plant growth and developmental processes. However, the characteristics and biological roles of the IDD gene family in tomato (Solanum lycopersicum) are still largely unexplored. In this study, 17 SlIDD genes were identified in the tomato genome and classified into seven subgroups according to the evolutionary relationships of IDD proteins. Analysis of exon-intron structures and conserved motifs reflected the evolutionary conservation of SlIDDs in tomato. Collinearity analysis revealed that segmental duplication promoted the expansion of the SlIDD family. Ka/Ks analysis indicated that SlIDD gene orthologs experienced predominantly purifying selection throughout evolution. The analysis of cis-acting elements revealed that the promoters of SlIDD genes contain numerous elements associated with light, plant hormones, and abiotic stresses. The RNA-seq data and qRT-PCR experimental results showed that the SlIDD genes exhibited tissue-specific expression. Additionally, Group A members from Arabidopsis thaliana and rice are known to play a role in regulating plant shoot gravitropism. QRT-PCR analysis confirmed that the expression level of SlIDD15 in Group A was high in the hypocotyls and stems. Subcellular localization demonstrated that the SlIDD15 protein was localized in the nucleus. Surprisingly, the loss-of-function of SlIDD15 by CRISPR/Cas9 gene editing technology did not display obvious gravitropic response defects, implying the existence of functional redundant factors within SlIDD15. Taken together, this study offers foundational insights into the tomato IDD gene family and serves as a valuable guide for exploring their molecular mechanisms in greater detail. |
| Keyword | IDD gene family SlIDD15 gravitropism Solanum lycopersicum |
| Subject Area | Biochemistry & Molecular Biology ; Chemistry |
| DOI | 10.3390/ijms251910422 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001334120200001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/14436 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Univ Sci & Technol China, Sch Life Sci, Div Life Sci & Med, Hefei 230027, Peoples R China 2.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, CAS Key Lab Top Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, 88 Xuefu Rd, Kunming 650223, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Wu, Huan,Liu, Mingli,Fang, Yuqi,et al. Genome-Wide Characterization of the INDETERMINATE DOMAIN (IDD) Zinc Finger Gene Family in Solanum lycopersicum and the Functional Analysis of SlIDD15 in Shoot Gravitropism[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2024,25(19):-. |
| APA | Wu, Huan.,Liu, Mingli.,Fang, Yuqi.,Yang, Jing.,Xie, Xiaoting.,...&Bai, Quanzi.(2024).Genome-Wide Characterization of the INDETERMINATE DOMAIN (IDD) Zinc Finger Gene Family in Solanum lycopersicum and the Functional Analysis of SlIDD15 in Shoot Gravitropism.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,25(19),-. |
| MLA | Wu, Huan,et al."Genome-Wide Characterization of the INDETERMINATE DOMAIN (IDD) Zinc Finger Gene Family in Solanum lycopersicum and the Functional Analysis of SlIDD15 in Shoot Gravitropism".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25.19(2024):-. |
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