| Rapid intracellular acidification is a plant defense response countered by the brown planthopper | |
| Jiang, Yanjuan3; Zhang, Xiao-Ya; Li, Shaoqin3,4; Xie, Yu-Cheng; Luo, Xu-Mei; Yang, Yongping3,4; Pu, Zhengyan; Zhang, Li5,6; Lu, Jia-Bao; Huang, Hai-Jian; Zhang, Chuan-Xi; He, Sheng Yang2,5,6 | |
| 2024 | |
| Source Publication | CURRENT BIOLOGY
 |
| ISSN | 0960-9822 |
| Volume | 34Issue:21Pages:- |
| Abstract | The brown planthopper (BPH) is the most destructive insect pest in rice. Through a stylet, BPH secretes a plethora of salivary proteins into rice phloem cells as a crucial step of infestation. However, how various salivary proteins function in rice cells to promote insect infestation is poorly understood. Among them, one of the salivary proteins is predicted to be a carbonic anhydrase ( Nilaparvata lugens carbonic anhydrase [NlCA]). The survival rate of the NlCA-RNA interference (RNAi) BPH insects was extremely low on rice, indicating a vital role of this salivary protein in BPH infestation. We generated NlCA transgenic rice plants and found that NlCA expressed in rice plants could restore the ability of NlCA- RNAi BPH to survive on rice. Next, we produced rice plants expressing the ratiometric pH sensor pHusion and found that NlCA- RNAi BPH induced rapid intracellular acidification of rice cells during feeding. Further analysis revealed that both NlCA- RNAi BPH feeding and artificial lowering of intracellular pH activated plant defense responses and that NlCA-mediated intracellular pH stabilization is linked to diminished defense responses, including reduced callose deposition at the phloem sieve plates and suppressed defense gene expression. Given the importance of pH homeostasis across the kingdoms of life, discovery of NlCA-mediated intracellular pH modulation uncovered a new dimension in the interaction between plants and piercing/sucking insect pests. The crucial role of NlCA for BPH infestation of rice suggests that NlCA is a promising target for chemical or trans-kingdom RNAibased inactivation for BPH control strategies in plants. |
| Subject Area | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology |
| DOI | 10.1016/j.cub.2024.09.039 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001351223700001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/14417 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Yunnan Univ, State Key Lab Conservat & Utilizat Bioresources Yu, Kunming 650091, Peoples R China 2.Zhejiang Univ, Inst Insect Sci, Hangzhou 310058, Peoples R China 3.Michigan State Univ, DOE Plant Res Lab, E Lansing, MI 48824 USA 4.Chinese Acad Sci, CAS Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Kunming 650223, Yunnan, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Duke Univ, Howard Hughes Med Inst, Durham, NC 27708 USA 7.Duke Univ, Dept Biol, Durham, NC 27708 USA 8.Ningbo Univ, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo, Peoples R China |
| Recommended Citation GB/T 7714 | Jiang, Yanjuan,Zhang, Xiao-Ya,Li, Shaoqin,et al. Rapid intracellular acidification is a plant defense response countered by the brown planthopper[J]. CURRENT BIOLOGY,2024,34(21):-. |
| APA | Jiang, Yanjuan.,Zhang, Xiao-Ya.,Li, Shaoqin.,Xie, Yu-Cheng.,Luo, Xu-Mei.,...&He, Sheng Yang.(2024).Rapid intracellular acidification is a plant defense response countered by the brown planthopper.CURRENT BIOLOGY,34(21),-. |
| MLA | Jiang, Yanjuan,et al."Rapid intracellular acidification is a plant defense response countered by the brown planthopper".CURRENT BIOLOGY 34.21(2024):-. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Rapid intracellular （4640KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment