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| Rapid DNA barcoding‐based fern and lycophyte inventories of protected areas—A pilot study to introduce a simple but effective protocol | |
| Liu, Hongmei; Chai, Yarong; Schneider, Harald | |
| 2024 | |
| Source Publication | Integrative Conservation
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| ISSN | 2770-9329 |
| Abstract | Abstract Recording inventories of species conserved in protected areas is a key step to evaluate the effectiveness of Kunming Montreal Global Biodiversity Framework (KM‐GBF) targets, such as the expansion of protected areas. The application of DNA barcoding facilitates the rapid production of enables to obtain rapid inventories with reduced reliance on taxon experts. These inventories aim not only to confirm existing records but also to minimize gaps in our knowledge of the distribution and taxonomy of species targeted for conservation through the implementation of protected areas. This pilot study introduces a simplified DNA barcoding pipeline as a reliable tool for recording fern and lycophyte species occurring in protected areas. The pipeline emphasizes limited and/or short training requirements, reducing the input required from taxon experts and maximizing shared benefits between conservationists and taxonomists. Despite using a single DNA barcoding region, 78% of the accessions were unambiguously identified to the species level. This applied approach not only confirmed previous records but also identified several previously overlooked species, either as newly recorded species conserved in the protected area or as species new to science. The pilot project effectively documented known species diversity and identified gaps in our taxonomic knowledge by discovering previously unknown and locally rare taxa. This rapid assessment enhances productive exchanges between conservation practitioners and taxon experts, with substantial benefits for both parties. 摘要 了解和认识保护区内的生物多样性是开展科学研究和保护的前提,也是实现昆明‐蒙特利尔公约目标的基础,但是分类学家人数和规模的不足是当前开展保护区生物多样性编目和分类研究的制约因素之一,而相对标准化的DNA条形码技术是一种不过度依赖分类学家的快速高效的研究手段。在保护区内开展深入的野外调查,并结合DNA条形码研究,不仅可以记录保护区内的常见物种,而且有助于发现珍稀物种或地区特有种。 本研究通过在西双版纳国家级自然保护区勐仑子片区的绿石林开展石松类和蕨类野外调查,并结合室内DNA条形码研究,对该保护区内的蕨类植物多样性进行编目和鉴定。我们的研究发现,本研究手段不仅能发现和记录大部分的常见物种,还记录到勐仑特有种1个、勐仑新纪录种1个和可能的新种1个。本研究的优势是,通过相对易培训和操作的DNA条形码技术,在不过度依赖类群专家的情况下,可以快速完成保护区的物种编目和鉴定,进而能够提升对保护区生物多样性的认知和物种保护。 本研究的结果表明,仅利用单个通用的DNA条形码片段(叶绿体rbcL片段)即可以实现对78%的物种的准确鉴定,通过结合标本检视和文献查阅,还记录到勐仑特有种1个、勐仑新纪录种1个和可能的新种1个。因此,本研究呼吁未来的保护区物种调查和生物多样性编目可以引入DNA条形码技术,以实现对保护区的生物多样性的认识和研究。【翻译:刘红梅】 Plain language summary Recording the biodiversity conserved in protected areas is a crucial cornerstone to assess their effectiveness. Unfortunately, assessing the diversity within protected areas has been a major challenge due to the scarcity of taxonomists who can provide reliable identification. Here, we introduce a simple DNA barcoding approach that can be established and carried out by conservation practitioners with minimal involvement of taxonomic experts. Notably, the records obtained through this approach can confirm the occurrence of previously recorded species, identify new species, and even detect gaps in our taxonomic knowledge. These multiple benefits were demonstrated through a pilot project that recorded the fern species diversity in a small conservation area in southwest China, utilizing a simplified DNA barcoding approach to minimize dependence on taxonomic experts. 通俗语言摘要 了解保护区内的生物多样性是开展研究和保护的前提,但是分类学家人数和规模的不足是当前开展保护区生物多样性编目和分类研究的制约因素之一。传统的生物多样性数据收集往往基于科研人员进行的科学考察,并主要依赖分类学家进行物种鉴定。在当前生物多样性丧失日益加剧的情况下,如何高效地开展生物类群的记录和研究成为日益紧迫的任务。DNA条形码技术是利用生物体DNA中一段保守片段对物种进行快速准确鉴定的技术,具有易操作、易标准化和相对客观的优势,分类学家可以用DNA条形码技术鉴定物种及物种间亲缘关系,修改已有的分类学结论。通过对年轻的非专业人员进行短期培训,开展DNA条形码研究,结合分类学家的最终把关和质量控制,可以实现对研究区域内生物多样性的快速编目和鉴定。因此,分类学家+DNA条形码技术的结合可以有效提升我们对地区生物多样性的认识,我们呼吁应在更大范围内推广和应用。 Practitioner points This highly simplified DNA barcoding protocol can be easily applied to address the species identification bottleneck. Communicating results with experts is crucial for confirming new species records and possibly identifying species that may be new to science. DNA barcoding improves the documentation of known species while also expanding the number of rare species known to occur in protected areas. 实践者要点 整合分类学家的专业知识和DNA条形码技术,能提高地区生物多样性编目的效率; 现代研究手段与科研人员专业知识的有机结合,有利于更全面地了解和认识生物多样性; DNA条形码技术不仅能够记录地区常见种,也有助于发现潜在的新物种。 |
| DOI | https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/inc3.60 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/14405 |
| Collection | Integrative Conservation |
| Affiliation | 1.Liu, Hongmei (Center for Integrative Conservation and Yunnan Key Laboratory for Conservation of Tropical Rainforests and Asian Elephants, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Menglun, Yunnan, China) 2.Chai, Yarong (Center for Integrative Conservation and Yunnan Key Laboratory for Conservation of Tropical Rainforests and Asian Elephants, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Menglun, Yunnan, China 3.School of Tea & Coffee, Pu'er University, Pu'er, Yunnan, China) 4.Schneider, Harald (Center for Integrative Conservation and Yunnan Key Laboratory for Conservation of Tropical Rainforests and Asian Elephants, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Menglun, Yunnan, China) |
| Recommended Citation GB/T 7714 | Liu, Hongmei,Chai, Yarong,Schneider, Harald. Rapid DNA barcoding‐based fern and lycophyte inventories of protected areas—A pilot study to introduce a simple but effective protocol[J]. Integrative Conservation,2024. |
| APA | Liu, Hongmei,Chai, Yarong,&Schneider, Harald.(2024).Rapid DNA barcoding‐based fern and lycophyte inventories of protected areas—A pilot study to introduce a simple but effective protocol.Integrative Conservation. |
| MLA | Liu, Hongmei,et al."Rapid DNA barcoding‐based fern and lycophyte inventories of protected areas—A pilot study to introduce a simple but effective protocol".Integrative Conservation (2024). |
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| Rapid DNA barcoding‐（2481KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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