| The MIO1-MtKIX8 module regulates the organ size in Medicago truncatula | |
Mao, Yawen1; Zhou, Shaoli1; Yang, Jing2; Wen, Jiangqi3; Wang, Dongfa4; Zhou, Xuan1; Wu, Xinyuan1; He, Liangliang; Liu, Mingli5; Wu, Huan; Yang, Liling; Zhao, Baolin; Tadege, Million3; Liu, Yu; Liu, Changning ; Chen, Jianghua1,2,6
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| 2023 | |
| Source Publication | PHYSIOLOGIA PLANTARUM
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| ISSN | 0031-9317 |
| Volume | 175Issue:5Pages:_ |
| Abstract | Plant organ size is an important agronomic trait tightly related to crop yield. However, the molecular mechanisms underlying organ size regulation remain largely unexplored in legumes. We previously characterized a key regulator F-box protein MINI ORGAN1 (MIO1)/SMALL LEAF AND BUSHY1 (SLB1), which controls plant organ size in the model legume Medicago truncatula. In order to further dissect the molecular mechanism, MIO1 was used as the bait to screen its interacting proteins from a yeast library. Subsequently, a KIX protein, designated MtKIX8, was identified from the candidate list. The interaction between MIO1 and MtKIX8 was confirmed further by Y2H, BiFC, split-luciferase complementation and pull-down assays. Phylogenetic analyses indicated that MtKIX8 is highly homologous to Arabidopsis KIX8, which negatively regulates organ size. Moreover, loss-of-function of MtKIX8 led to enlarged leaves and seeds, while ectopic expression of MtKIX8 in Arabidopsis resulted in decreased cotyledon area and seed weight. Quantitative reverse-transcription PCR and in situ hybridization showed that MtKIX8 is expressed in most developing organs. We also found that MtKIX8 serves as a crucial molecular adaptor, facilitating interactions with BIG SEEDS1 (BS1) and MtTOPLESS (MtTPL) proteins in M. truncatula. Overall, our results suggest that the MIO1-MtKIX8 module plays a significant and conserved role in the regulation of plant organ size. This module could be a good target for molecular breeding in legume crops and forages. |
| Subject Area | Plant Sciences |
| DOI | 10.1111/ppl.14046 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001095996200013 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/13886 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, CAS Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Kunming, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Yunnan Univ, Sch Ecol & Environm Sci, Kunming, Peoples R China 4.Oklahoma State Univ, Inst Agr Biosci, Ardmore, OK USA 5.Univ Sci & Technol China, Sch Life Sci, Hefei, Peoples R China 6.Southwest Forestry Univ, Kunming, Peoples R China 7.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, 88 Xuefu Rd, Kunming 650223, Yunnan, Peoples R China |
| Recommended Citation GB/T 7714 | Mao, Yawen,Zhou, Shaoli,Yang, Jing,et al. The MIO1-MtKIX8 module regulates the organ size in Medicago truncatula[J]. PHYSIOLOGIA PLANTARUM,2023,175(5):_. |
| APA | Mao, Yawen.,Zhou, Shaoli.,Yang, Jing.,Wen, Jiangqi.,Wang, Dongfa.,...&Chen, Jianghua.(2023).The MIO1-MtKIX8 module regulates the organ size in Medicago truncatula.PHYSIOLOGIA PLANTARUM,175(5),_. |
| MLA | Mao, Yawen,et al."The MIO1-MtKIX8 module regulates the organ size in Medicago truncatula".PHYSIOLOGIA PLANTARUM 175.5(2023):_. |
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