| GhWRKY33 negatively regulates jasmonate-mediated plant defense to Verticillium dahliae | |
Ji, Yunrui2; Mou, Minghui2; Zhang, Huimin; Wang, Ruling; Wu, Songguo; Jing, Yifen2; Zhang, Haiyan2; Li, Lanxin2; Li, Zhifang3; Chen, Ligang1
| |
| 2023 | |
| Source Publication | PLANT DIVERSITY
 |
| ISSN | 2096-2703 |
| Volume | 45Issue:3Pages:337-346 |
| Abstract | Verticillium wilt, caused by Verticillium dahliae, seriously restricts the yield and quality improvement of cotton. Previous studies have revealed the involvement of WRKY members in plant defense against V. dahliae, but the underlying mechanisms involved need to be further elucidated. Here, we demon-strated that Gossypium hirsutum WRKY DNA-binding protein 33 (GhWRKY33) functions as a negative regulator in plant defense against V. dahliae. GhWRKY33 expression is induced rapidly by V. dahliae and methyl jasmonate, and overexpression of GhWRKY33 reduces plant tolerance to V. dahliae in Arabidopsis. Quantitative RT-PCR analysis revealed that expression of several JA-associated genes was significantly repressed in GhWRKY33 overexpressing transgenic plants. Yeast one-hybrid analysis revealed that GhWRKY33 may repress the transcription of both AtERF1 and GhERF2 through its binding to their pro-moters. Protein-protein interaction analysis suggested that GhWRKY33 interacts with G. hirsutum JASMONATE ZIM-domain protein 3 (GhJAZ3). Similarly, overexpression of GhJAZ3 also decreases plant tolerance to V. dahliae. Furthermore, GhJAZ3 acts synergistically with GhWRKY33 to suppress both AtERF1 and GhERF2 expression. Our results imply that GhWRKY33 may negatively regulate plant toler-ance to V. dahliae via the JA-mediated signaling pathway. Copyright & COPY; 2022 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY -NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Keyword | GhWRKY33 Verticillium dahliae Jasmonate acid GhJAZ3 Defense response |
| Subject Area | Plant Sciences |
| DOI | 10.1016/j.pld.2022.04.0012468-2659 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001014956800001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/13654 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Chinese Acad Sci, CAS Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Menglun 666303, Yunnan, Peoples R China 2.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Menglun 666303, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Henan Univ, Sch Life Sci, Kaifeng 475004, Peoples R China |
| Recommended Citation GB/T 7714 | Ji, Yunrui,Mou, Minghui,Zhang, Huimin,et al. GhWRKY33 negatively regulates jasmonate-mediated plant defense to Verticillium dahliae[J]. PLANT DIVERSITY,2023,45(3):337-346. |
| APA | Ji, Yunrui.,Mou, Minghui.,Zhang, Huimin.,Wang, Ruling.,Wu, Songguo.,...&Chen, Ligang.(2023).GhWRKY33 negatively regulates jasmonate-mediated plant defense to Verticillium dahliae.PLANT DIVERSITY,45(3),337-346. |
| MLA | Ji, Yunrui,et al."GhWRKY33 negatively regulates jasmonate-mediated plant defense to Verticillium dahliae".PLANT DIVERSITY 45.3(2023):337-346. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| GhWRKY33 negatively （2772KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment