| Hypoglycemic and Antioxidant Properties of Extracts and Fractions from Polygoni Avicularis Herba | |
| Zhang, Kun; Han, Mei1; Zhao, Xia; Chen, Xuelin1; Wang, Hanlei1; Ni, Jiyan1; Zhang, Yumei1 | |
| 2022 | |
| Source Publication | MOLECULES
 |
| ISSN | 1420-3049 |
| Volume | 27Issue:11Pages:- |
| Abstract | Our research focused on the hypoglycemic capability and the possible mechanisms of extract and fractions from Polygoni Avicularis Herba (PAH) based on alpha-glucosidase, alpha-amylase inhibition assays, glucose uptake experiment, HPLC-MS analysis, and molecular docking experiment. In addition, DPPH, ABTS, and FRAP assays were used for determining the antioxidant capability. The results of total flavonoids and phenolics contents showed that ethyl acetate fraction (EAF) possessed the highest flavonoids and phenolics with values of 159.7 +/- 2.5 mg rutin equivalents/g and 107.6 +/- 2.0 mg galic acid equivalents/g, respectively. The results of in vitro hypoglycemic activity showed that all samples had effective alpha-glucosidase inhibition capacities, and EAF possessed the best inhibitory effect with IC50 value of 1.58 +/- 0.24 mu g/mL. In addition, n-butanol fraction (NBF) significantly promoted the glucose uptake rate of 3T3-L1 adipocytes. HPLC-MS analysis and molecular docking results proved the interactions between candidates and alpha-glucosidase. The results of antioxidation capacities showed that EAF possessed the best antioxidation abilities with DPPH, ABTS, and FRAP. In summary, the hypoglycemic activity of PAH might be related to the inhibition of alpha-glucosidase (EAF > PEF > NBF) and the promotion of glucose uptake in 3T3-L1 adipocytes (NBF). Simultaneously, the antioxidation capacity of PAH might be related to the abundant contents of flavonoids and other phenolics (EAF > PEF > NBF). |
| Keyword | Polygoni Avicularis Herba hypoglycemic antioxidant flavonoids phenolics molecular docking |
| Subject Area | Biochemistry & Molecular Biology ; Chemistry |
| DOI | 10.3390/molecules27113381 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000809071300001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/13178 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Chinese Acad Sci, Key Lab Trop Plant Resource & Sustainable Use, Xishuangbanna Trop Bot Garden, Kunming 650223, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Kun,Han, Mei,Zhao, Xia,et al. Hypoglycemic and Antioxidant Properties of Extracts and Fractions from Polygoni Avicularis Herba[J]. MOLECULES,2022,27(11):-. |
| APA | Zhang, Kun.,Han, Mei.,Zhao, Xia.,Chen, Xuelin.,Wang, Hanlei.,...&Zhang, Yumei.(2022).Hypoglycemic and Antioxidant Properties of Extracts and Fractions from Polygoni Avicularis Herba.MOLECULES,27(11),-. |
| MLA | Zhang, Kun,et al."Hypoglycemic and Antioxidant Properties of Extracts and Fractions from Polygoni Avicularis Herba".MOLECULES 27.11(2022):-. |
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| Hypoglycemic and Ant（2667KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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