| OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2 | |
Li, Chenyang1; Li, Yang1; Xu, Peng1; Liang, Gang1
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| 2022 | |
| Source Publication | PLANT JOURNAL
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| ISSN | 0960-7412 |
| Volume | 111Issue:4Pages:966-978 |
| Abstract | Iron (Fe) is crucial for crop productivity and quality. However, Fe deficiency is prevalent worldwide, particularly in alkaline soil. Plants have evolved sophisticated mechanisms to withstand Fe-deficient conditions. Oryza sativa IRON-RELATED BHLH TRANSCRIPTION FACTOR 3 (OsIRO3/OsbHLH63) has been identified as a negative regulator of Fe deficiency response signaling; however, the underlying mechanism remains unclear. In the present study, we constructed two iro3 mutants, which developed leaves with necrotic lesions under Fe-deficient conditions. Loss-of-function of OsIRO3 caused upregulation of Fe deficiency-associated genes in the root. Fe concentration measurements showed that the iro3 mutants had increased shoot Fe concentration only under Fe-deficient conditions. Further analysis revealed that OsIRO3 directly regulated the expression of IRON-RELATED BHLH TRANSCRIPTION FACTOR 2 (OsIRO2), which encodes a positive regulator of the Fe uptake system. Further investigation demonstrated that OsIRO3 interacted with POSITIVE REGULATOR OF IRON HOMEOSTASIS 1(OsPRI1) and OsPRI2, and. OsIRO3 repressed their transcription activation towards OsIRO2. OsIRO3 contains an EAR motif, which recruits the TOPLESS/TOPLESS-RELATED (OsTPL/OsTPRs) corepressors. Mutation of the EAR motif attenuated the repression ability of OsIRO3. This work sheds light on the molecular mechanism by which OsIRO3 modulates Fe homeostasis in rice. |
| Keyword | iron OsIRO3 OsIRO2 OsFIT OsPRI1 OsPRI2 |
| Subject Area | Plant Sciences |
| DOI | 10.1111/tpj.15864 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000818900600001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/13157 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Chinese Acad Sci, Innovat Acad Seed Design, Xishuangbanna Trop Bot Garden, CAS Key Lab Trop Plant Resources & Sustainable Us, Kunming 650223, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Chenyang,Li, Yang,Xu, Peng,et al. OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2[J]. PLANT JOURNAL,2022,111(4):966-978. |
| APA | Li, Chenyang,Li, Yang,Xu, Peng,&Liang, Gang.(2022).OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2.PLANT JOURNAL,111(4),966-978. |
| MLA | Li, Chenyang,et al."OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2".PLANT JOURNAL 111.4(2022):966-978. |
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| OsIRO3 negatively re（3125KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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