| Molecular evolution and functional characterization of chitinase gene family in Populus trichocarpa | |
| Zhang, Yuan-Jie; Ren, Lin-Ling; Lin, Xiao-Yang; Han, Xue-Min; Wang, Wei1; Yang, Zhi-Ling | |
| 2022 | |
| Source Publication | GENE
 |
| ISSN | 0378-1119 |
| Volume | 822Issue:xPages:- |
| Abstract | Chitinases, the chitin-degrading enzymes, have been shown to play important role in defense against the chitincontaining fungal pathogens. In this study, we identified 48 chitinase-coding genes from the woody model plant Populus trichocarpa. Based on phylogenetic analysis, the Populus chitinases were classified into seven groups. Different gene structures and protein domain architectures were found among the seven Populus chitinase groups. Selection pressure analysis indicated that all the seven groups are under purifying selection. Phylogenetic analysis combined with chromosome location analysis showed that Populus chitinase gene family mainly expanded through tandem duplication. The Populus chitinase gene family underwent marked expression divergence and is inducibly expressed in response to treatments, such as chitosan, chitin, salicylic acid and methyl jasmonate. Protein enzymatic activity analysis showed that Populus chitinases had activity towards both chitin and chitosan. By integrating sequence characteristic, phylogenetic, selection pressure, gene expression and protein activity analysis, this study shed light on the evolution and function of chitinase family in poplar. |
| Keyword | Chitinase Functional divergence Poplar Stress response Tandem duplication |
| Subject Area | Genetics & Heredity |
| DOI | 10.1016/j.gene.2022.146329 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000776237900008 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/13068 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.[Zhang, Yuan-Jie 2.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China 3.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 5.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China 6.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Key Lab Trop Forest Ecol, Mengla 666303, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Yuan-Jie,Ren, Lin-Ling,Lin, Xiao-Yang,et al. Molecular evolution and functional characterization of chitinase gene family in Populus trichocarpa[J]. GENE,2022,822(x):-. |
| APA | Zhang, Yuan-Jie,Ren, Lin-Ling,Lin, Xiao-Yang,Han, Xue-Min,Wang, Wei,&Yang, Zhi-Ling.(2022).Molecular evolution and functional characterization of chitinase gene family in Populus trichocarpa.GENE,822(x),-. |
| MLA | Zhang, Yuan-Jie,et al."Molecular evolution and functional characterization of chitinase gene family in Populus trichocarpa".GENE 822.x(2022):-. |
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| Molecular evolution （4418KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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