| Functional characterization, antimicrobial effects, and potential antibacterial mechanisms of new mastoparan peptides from hornet venom (Vespa ducalis, Vespa mandarinia, and Vespa affinis) | |
Wen, Xinxin2; Gongpan, Pianchou1; Meng, Yichuan2; Nieh, James C.; Yuan, Hongling3; Tan, Ken
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| 2021 | |
| Source Publication | TOXICON
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| ISSN | 0041-0101 |
| Volume | 200Issue:xPages:48-54 |
| Abstract | Antibiotic-resistant bacteria are a major threat to global public health, and there is an urgent need to find effective, antimicrobial treatments that can be well tolerated by humans. Hornet venom is known to have antimicrobial properties, and contains peptides with similarity to known antimicrobial eptides (AMPs), mastoparans. We identified multiple new AMPs from the venom glands of Vespa ducalis (U-VVTX-Vm1a, U-VVTX-Vm1b, and U-VVTX-Vm1c), Vespa mandarinia (U-VVTX-Vm1d), and Vespa affinis (U-VVTX-Vm1e). All of these AMPs have highly similar sequences and are related to the toxic peptide, mastoparan. Our newly identified AMPs have a-helical structures, are amphiphilic, and have antimicrobial properties. Both U-VVTX-Vm1b and U-VVTX-Vm1e killed bacteria, Staphylococcus aureus ATCC25923 and Escherichia colt ATCC25922, at the concentrations of 16 mu g/mL and 32 mu g/mL, respectively. None of the five AMPs exhibited strong toxicity as measured via their hemolytic activity on red blood cells. U-VVTX-Vm1b was able to increase the permeability of E. coli ATCC25922 and degrade its genomic DNA. These results are promising, demonstrate the value of investigating hornet venom as an antimicrobial treatment, and add to the growing arsenal of such naturally derived treatments. |
| Keyword | Antimicrobial peptides Drug-resistance alpha-helical structure Amphiphilic Antibiotic |
| Subject Area | Pharmacology & Pharmacy ; Toxicology |
| DOI | 10.1016/j.toxicon.2021.07.001 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000691881600008 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/12355 |
| Collection | 2012年后新成立研究组 |
| Affiliation | 1.Chinese Acad Sci, CAS Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Kunming 650000, Yunnan, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Trop Plant Resources & Sustainable Us, Xishuangbanna Trop Bot Garden, Kunming 650000, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Kunming Med Univ, Affiliated Hosp 1, Kunming 650000, Yunnan, Peoples R China 5.Nieh, James C.] Univ Calif San Diego, Div Biol Sci, Sect Ecol Behav & Evolut, La Jolla, CA 92093 USA |
| Recommended Citation GB/T 7714 | Wen, Xinxin,Gongpan, Pianchou,Meng, Yichuan,et al. Functional characterization, antimicrobial effects, and potential antibacterial mechanisms of new mastoparan peptides from hornet venom (Vespa ducalis, Vespa mandarinia, and Vespa affinis)[J]. TOXICON,2021,200(x):48-54. |
| APA | Wen, Xinxin,Gongpan, Pianchou,Meng, Yichuan,Nieh, James C.,Yuan, Hongling,&Tan, Ken.(2021).Functional characterization, antimicrobial effects, and potential antibacterial mechanisms of new mastoparan peptides from hornet venom (Vespa ducalis, Vespa mandarinia, and Vespa affinis).TOXICON,200(x),48-54. |
| MLA | Wen, Xinxin,et al."Functional characterization, antimicrobial effects, and potential antibacterial mechanisms of new mastoparan peptides from hornet venom (Vespa ducalis, Vespa mandarinia, and Vespa affinis)".TOXICON 200.x(2021):48-54. |
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| Functional character（3144KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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