| POSITIVE REGULATOR OF IRON HOMEOSTASIS1, OsPRI1, Facilitates Iron Homeostasis | |
Zhang, Huimin; Li, Yang; Yao, Xiani; Liang, Gang ; Yu, Diqiu
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| 2017 | |
| Source Publication | PLANT PHYSIOLOGY
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| Volume | 175Issue:1Pages:543-554 |
| Abstract | Oryza sativa HEMERYTHRIN MOTIF-CONTAINING REALLY INTERESTING NEW GENE AND ZINC-FINGER PROTEIN1 (OsHRZ1) is a putative iron-binding sensor. However, it is unclear how OsHRZ1 transmits signals. In this study, we reveal that POSITIVE REGULATOR OF IRON HOMEOSTASIS1 (OsPRI1) interacts with OsHRZ1. A loss-of-function mutation to OsPRI1 increased the sensitivity of plants to Fe-deficient conditions and down-regulated the expression of Fe-deficiency-responsive genes. Yeast one-hybrid and electrophoretic mobility shift assay results suggested that OsPRI1 binds to the OsIRO2 and OsIRO3 promoters. In vitro ubiquitination experiments indicated that OsPRI1 is ubiquitinated by OsHRZ1. Cell-free degradation assays revealed that the stability of OsPRI1 decreased in wild-type roots but increased in the hrz1-2 mutant, suggesting OsHRZ1 is responsible for the instability of OsPRI1. The hrz1-2 seedlings were insensitive to Fe-deficient conditions. When the pri1-1 mutation was introduced into hrz1-2 mutants, the pri1hrz1 double mutant was more sensitive to Fe deficiency than the hrz1-2 mutant. Additionally, the expression levels of Fe-deficiency-responsive genes were lower in the hrz1pri1 double mutant than in the hrz1-2 mutant. Collectively, these results imply that OsPRI1, which is ubiquitinated by OsHRZ1, mediates rice responses to Fe deficiency by positively regulating OsIRO2 and OsIRO3 expression as part of the OsHRZ1-OsPRI1- OsIRO2/3 signal transduction cascade. |
| Department | 其他 |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | https://ir.xtbg.ac.cn/handle/353005/10593 |
| Collection | 植物分子生物学研究组 2012年后新成立研究组 |
| Recommended Citation GB/T 7714 | Zhang, Huimin,Li, Yang,Yao, Xiani,et al. POSITIVE REGULATOR OF IRON HOMEOSTASIS1, OsPRI1, Facilitates Iron Homeostasis[J]. PLANT PHYSIOLOGY,2017,175(1):543-554. |
| APA | Zhang, Huimin,Li, Yang,Yao, Xiani,Liang, Gang,&Yu, Diqiu.(2017).POSITIVE REGULATOR OF IRON HOMEOSTASIS1, OsPRI1, Facilitates Iron Homeostasis.PLANT PHYSIOLOGY,175(1),543-554. |
| MLA | Zhang, Huimin,et al."POSITIVE REGULATOR OF IRON HOMEOSTASIS1, OsPRI1, Facilitates Iron Homeostasis".PLANT PHYSIOLOGY 175.1(2017):543-554. |
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| POSITIVE REGULATOR O（1727KB） | 开放获取 | CC BY-NC-SA | View Download | |||
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